Characterizing The Effluents Of Anaerobic Methane-producing Reactor Using Molecular Spectroscopy

The biological wasterwater treatment reactors with various pollutants arecomplex systems.Conventional chemical analysis can not meet the requirement ofreal-time and on-line monitoring of bioreactors.Spectrometry method is a regent-freeone,and can be performed on-line and insitu.Thus,it is a promising methed formonitorng the bioreactor.In this study the anaerobic menthanogenesis reactor was the research object,usingthe chemometrical method to decompose the spectra，extracting spectral informationfrom anaerobic reactor effluent samples.The information was related to the bioreactorsprocess parameters.The objective of this work was to develop a new methed ofcharacterizing the parameter and status of anaerobic methane ractor,based on spectra.The main staps are as follow:operating the reactorâ†'characterizing the effluents ofanaerobic methane-producing reactor using the spectroscopyâ†'analyzing the spectrumdataâ†'reactor near-infraed spectrum and volatile fatty acids modeling analysisâ†'runningstate associated with changes in the fluorescence spectrum analysis. using the spectrumanalysis theory and method to study the near-infraed spectrum and fluorescencespectrum of anaerobic reactor effluent samples.Chemometrical method was adopted tobuild the relational model of near-infraed spectrum and volatile fatty acids of anaerobicmethane reactor effluent.Relevance of anaerobic reactor state and EEM fluorescencespectra was analyzed.The near-infrared spectra of mixed volatile fatty acid (VFA) and sucrosesolution were analyzed using partial least squares method. Results showed that thecorrelation coefficients of the predicted value of acetic acid, propionic acid and butyricacid and the actual value were0.9935,0.9907,0.9952, respectively. The predictionconcentration was correlated well with the real concentration. Based on the aboveresults, effluents from the anaerobic methanogenic reactor was characterized with thenear infrared spectral during its starting process, and combined with the results of gaschromatography, the quantitative analysis model was established with partial leastsquares method. The results showed that the established model could reflect thevariation of the volatile fatty acids concentration of the effluents from the anreactor.Characterizing the effluents of anaerobic methane-producing reactor by usingthe EEM fluorescence spectra,the normal operation of the reactor that the effluent of fluorescent substances mainly include protein, coenzyme NADH and coenzyme F420,when the methanogenesis is restrained, the water coenzyme F420was out of the reactor,so fluorescent material mainly include proteins and coenyme NADH. EEM fluorescecespectra can reflect the condition of anreactor and the biological activities ofmethanogens, the concentration of the fluorescent substance between the station of thereactor has the close inner link, the fluorescent material of fluorescence intensity and theconcentration of the fluorescent substance of protein, coenzyme NADH and coenzymeF420, can reflect the activity of microorganisms in the reactor,and to judge the runningstate of reactor, it plays an important role in the reactor monitoring...