The Study On Tissue Culture And Clone Establishment Of Artemisia Argyi

Artemisia argyi is the perennial herb of the genus Artemisia in the famliy Compositae. It is a kind of natural herbal medicine which combines medical value and food nutritive value together. Moreover, it has a close connection with Dragon Boat Festival which is a traditional festival of China. Because of people collecting plenty of Artemisia argyi for long term as medicine, as well as excessive picking before Dragon Boat Festival, in the recent years, the wild resources of Artemisia argyi has been reduced rapidly, even in imminent danger exterminates. In order to protect wild resources and meet the need of people, the study focused on the tissue culture and clone establishment of Artemisia argyi. Systematic studies were done on different factors which affecting the process of tissue culture of Artemisia argyi. According to the studies, the optimum media for callus induction, callus differentiation, growing point differentiation and rooting were identified and finally the asexual regeneration system of Artemisia argyi was established successfully. The study laid the foundation of the technology to realize the protection of wild resources, the need of seedlings and the establishment of the gene pool of Artemisia argyi.The main contents and results of the study were shown as follows:1. The cultivation of callus induction and subcultureThe results showed that the most suitable explant for callus induction was the aseptic tender stem; the ideal medium for callus induction was MS+2,4-D2.0mg·L-1sucrose30g-L"1, and this kind of medium applied to subculture of induced callus as well.2. The cultivation of callus differentiationThe results demonstrated that MS+6-BA1.5mg-L-1+IAA0.3mg·L-1+sucrose30g·L-1was the optimum medium for callus differentiation, the differentiation rate was51.6%, and one callus could differentiate3.2buds on average.3. The cultivation of growing point differentiationThe results manifested that the optimum medium for growing point differentiation was MS+NAA1.0mg·L-1+GAs0.5mg·L-1-BA3.0mg·L-1+sucrose30g·L-1, in this kind of medium, the differentiation rate was93.3%and one growing point could differentiate5.3buds on average.4. The cultivation of rooting and subcultureThe results demonstrated that the optimum medium for rooting of adventitious buds was1/4MS+IAA0.2mg·L-1+sucrose20g·L-1;the optimum medium for rooting of shoot buds was N6+IAA0.2mg·L-!+sucrose20g·L-1;1/4MS+IAA0.2mg·L-1+sucrose20g·L-1was the ideal medium for rooting sbuculture. The cultivation of rooting and subculture was suitable to cultivate under homothermal illumination. In the process of cultivation of rooting subculture, insert the plant morphology bottom into medium could achieve the best result. Subculture for25days could get a generation of plantlets and the average propagation coefficient of per-generation was3.4.5. Plantlet transplanting colonizationGarden soil was chosen as transplanting substrate. The survival rate of transplanted tube seedlings could be up to100%. Colonization of plantlets grew vigorously and maintained all biological traits which wild plant had.