| Chicken’s body type could be Undersized with some genes’effection,this chickens called Dwarf chickens.Dwarf Parent breeders could save Diets, Almost didn’t produce the double yellow eggs,Fertilization rate and Hatchability were2%higher than normal chicken,and The rearing space occupied smaller.the production performance would not be affected when it hybridization with normal cock.Dwarf chicken was The direction of development of broiler breeders.neuropeptide Y was a Peptide with36Amino acids,it widely distributed In Hypothalamus and was one of the The highest neuropeptide content of that Central. nervoustissue.NPY hormone acting on Hypothalamic-Pituitary-Gonadalaxis(HPG),and using Sex hormone-dependent manner affect the sexual maturation startup. Currently,NPY gene has been considered to be one of the candidate genes associated with poultry breeding performance.Currently,Breeder main goal is to reduce the cost of rearing,Improve product quality and Create brand. Therefore, the study by comparison of three kinds of chicken B hen’s(Tequ dwarf Cyan-Shank Partridge chicken),C hen’s(Yuxi chicken bred after with Sanhuang chicken hybrid),and BC hen’s(Band C hybrid) egg Performance,acquire three hen’s Basis date of egg Performance.and Combined with molecular means,using Neuropeptide Y (NPY) gene as candidate genes for affecting chicken’s reproductive traits.,By PCR-SSCP analysis of chicken reproductive traits and the NPY gene polymorphism’s association.Aims Further explore the relationship between the NPY gene with the chicken breeding performance,for accumulation of basic data for molecular-assisted selection of quality broiler breeding traits.the result showed that:1By egg comparison of the performance of three kinds of hens,we get that B’s individual egg production was lower than C and BC’s in40weeks,C and BC’s individual egg production in40weeks were not differ significantly(P>0.05); the C’s and BC’s body weight at first egg and weight of the first egg were not differ significantly(P>0.05),and had Significant difference with C’s body weight at first egg and weight of the first egg.B’s age at first egg and the group body weight at first egg were significant difference with C’s(P<0.01),and differ significantly with BC’s (P<0.05), C’s age at first egg and the group body weight at first egg were differ significantly with BC’s(P<0.05).2Via PCR-SSCP Analysis,we acquired that a SNP (A629G)loci in B’s,C’s,and BC’s NPY gene,this loci change the Base A to Base Qchange the Asp to Gly.There three Genotype in this loci,AA, AB and BB,two Alleles A and B.there were no BB genotype in C’s and BC’s NPY gene.B’s AA,AB,BB Genotype frequencies were0.6082,0.3196and0.0722,A allele frequency is0.7680and B allele frequency is0.2320;C’s AA,AB Genotype frequencies were0.5918,0.4082,A allele frequency is0.7959and B allele frequency is0.2041;BC’s AA,AB Genotype frequencies were0.5579,0.4421,A allele frequency is0.7789and B allele frequency is0.2211.B hen P>0.05,so B’s NPY was in Hardy-Weinberg equilibrium state;C’s and BC’s0.01<P<0.05,so they are NPY was not in Hardy-Weinberg equilibrium state.three Group all in moderately polymorphic information content(0.25<PIC<0.5).3According to the detected genotype,Split290hen’s40weeks Individual egg production which have Egg production records.The res μ Lts showed that:Least squares mean of Homozygous genotype Individual egg production were higher than Heterozygous,AA>AB, BB>AB, B’s and C’s Homozygous genotype Individual egg production were differ significantly with Heterozygous(P<0.05),B’s Individual egg production of AA and BB were not differ significantly(P>0.05).Different genotype of three kinds of hens’ weight of the first egg,body weight at first egg and age at first egg were not differ significantly(P>0.05). |
