| 12swines are randomly divided into two groups,6for each group. Oral administration (gavage) is conducted to the swine with each single dose10mg·kg-1, the control group takes Enrofloxacin. The Enrofloxacin concentrations in blood at different intervals were detected by HPLC. The pharmacokinetic parameter were calculated with3P97program software processing Blood concentration-time data. The result shows that after orally taking Enrofloxacin powder, Enrofloxacin in swine body fitted to one compartment open model, here are the main pharmacokinetic parameters:Tmax (4.55±0.74) h, Cmax (1.08±0.25)μg·mL-1, T1/2ke (4.82±1.10) h, AUC (14.67±4.80) mg·h·mL-1. After orally taking Enrofloxacin, Enrofloxacin in swine body fitted to one compartment open model, here are the main pharmacokinetic parameters:Tmax (1.86±0.73) h, Cmax (1.79±0.52)μg·mL-1, T1/2ke (4.29±1.38) h, AUC (15.26±5.73) mg·h·mL-1. By comparing the medicine generation parameters of the two drugs it shows that compared with Enrofloxacin, in swine body Enrofloxacin powder is slower absorpted and has longer Tmax and elimination half life, the area under the concentration-time curve of the two drugs are nearly the same. The relative bioavailability of orally taking Enrofloxacin powder is96.13%. The findings of the study suggest that Enrofloxacin powder has the advantages of good absorption and high bioavailability.In vitro minimum inhibition concentration of Enrofloxacin powder against Actinobacillus Pleuropneumoniae which will be used to infect swines is=0.06μg·mL-1by trace broth dilution method. On the foundation of Enrofloxacin powder study and combined with the minimal inhibitory concentration of Enrofloxacin to Infectious pleuropneumonia actinobacillus, this study protocols2.5,5and10mg-kg-1as the concentration of Enrofloxacin Enrofloxacin powder to treat swine infectious pleuropneumonia, and successfully establishes artificial infection model of Swine Infectious Pleuropneumonia by intranasal inoculating Actinobacillus Pleuropneumon-iae.60pigs are grouped into6equally-sized groups, they are high dose group, medial dose group, low dose group, control group, Positive control group and negative control group. After artificial infected by Swine Infectious Pleuropneumonia, the pigs are treated by orally taken Enrofloxacin powder and observed for15days.6～48h after infection the mean body temperatures of Infection groups are apparently higher than drug treatment groups and healthy control group. The cure rates and the weight gain rates of high dose group, medial dose group and drug treatment groups are significantly higher than low dose group and Positive control group (P<0.01), while the cure rates and the weight gain rates of high dose group and medial dose group show no significant difference compared with the control group (P>0.05). It has remarkable curative effect to swine Infectious Pleuropneumonia by orally taking Enrofloxacin powder5～10mg·kg-1each time twice a day, continuously taking for5days.This study also evaluates the safety of Enrofloxacin powder being orally taken by swine the targeted animals based on VICH veterinary drug target animal security research guiding principle and the ministry of agriculture Veterinary Chemicals Safety Pharmacology Experiment Guiding Principle.24healthy pigs are randomly grouped in to4groups, they are a control group and three medicated groups orally taking drug twice a day for continuously for15days with the doses50mg·kg-1(5times the recommended dose),30mg·kg-1(3times the recommended dose),10mg·kg-1(the recommended dose) relatively. The adverse drug reactions are not observed during administration periods, the blood routine indexes such as red blood cell, platelet, hemoglobin and hematocrit have no significant differences between the periods before and after drug taking, and also does the biochemical index. The pathology of main visceral biopsy does not show any obvious pathological changes. There are no significant differences in the main viscera coefficients between control group and the different-dose-drug taking groups. This conclusion suggests that orally taking Enrofloxacin powder in the dose range of10mg·kg-1～50mg·kg-1has neither noticeable influence to swine blood physiological and biochemical parameter and organization structure nor toxic reaction. |
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