| Acidosis is a metabolic disorder in ruminants, which caused huge econmic losses in dairy industry. To explore whether the acarbose has the potential to minimize rumen acidosis, three experiments were conducted in this thesis. First, we investigated the effect of acarbose addition on mixed rumen micro-organisms fermentation in in vitro batch cultures. Second, we evaluated the effects of acarbose addition on the ruminal micro-organism in vitro batch cultures using the sorghum, corn, barley and wheat as substate and with a mixed diet charactized by richful nutition. Finally, we studied the effect of acarbose on the ruminal metabolism and blood serum indexes of goat. This thesis was described in the following three sections.In the first section, the effects of acarbose addition on in vitro rumen fermentation characterizes of ruminal bacteria was studied using the batch culture techniques. The acarbose was added to achieve final acarbose concentrations of0,0.067,0.13and0.26mg/mL. The diets consisted of the0.48g grinding concentration (corn:soybean meal=7:3) and0.12g grinding chinless. Control bottles had the same ingredients except no acarbose. Both acarbose treatment and the control had four replicate bottles. The results showed that during24h incubations, acarbose addition increased the pH (P<0.01) and the ratio of the acetate to propionate (P<0.05), and decreased the propionate, butyrate production (P<0.05) and total gas production (P<0.01), decreased the lactate production in the trend at a same time (P=0.099). There is no significant difference of NH3-N, isobutyrate, valerate, acetate, isovalerate and TVFA production between the control and the treatment. The results suggested that acarbose addition can decrease the fermentation activity effectively and has the potential to prevent the rumen acidosis.In the second section, this experiment was conducted to investigate the effect of acarbose addition(0,0.1,0.2and0.4mg/mL) on the fermentation characterics in in vitro batch cultures using the sorghum, corn, barley and wheat as substate and with a mixed diet charactized by richful nutition. The results showed that acarbose addition increased the pH, cumulative gas production, the ratio of the ratio of acetate to propionate, NH3-N and butyrate, propionate and valerate production (P<0.01) and decreased the lactate concentration (P<0.01) with richful nutrition diet.There is no significant difference on acetate, isobutyrate, isovalerate and TVFA production between the control and the acarbose treatment. While the sorghum, corn, barley and wheat were used as substate, the results showed that acarbose supplement increased pH, NH3-N, lactate production and the ratio of the acetate to propionate (P<0.01), and decreased acetate, propionate, butyrate and TVFA production (P<0.01).The results suggested that acarbose had a significant beneficial effect on the pH and ratio of acetate to propionate, therefore, acarbose could be used as a potential additive for ruminants.In the third section, four cannulated goats were used in a4×4Latin square design to investigate the effect of acarbose addition (0,15,30and60mg/d) on ruminal metabolism and the blood serum indexes of goat. The results showed that acarbose addition improved the pH of rumen fluid in the trend (P=0.094), and reduced the NH3-N production, propionate, butyrate and lactate production (P<0.05), and improved the ratio of acetate to propionate (P<0.01). There is no significant difference on acetate, isobutyrate, isovalerate, valerate and TVFA production between the control and the acarbose treatment (P>0.10). Acarbose addition did not influence the serum of concentration of lactate, glucose, urea N and triglyceride (P>0.10), while reduced the concentration of total protein (P<0.05) and improved the cholesterol concentration in the trend at a same time (P=0.059). The results indicated that acarbose addition had a benefited effect on improving rumen fermentation pattern and preventing rumen acidosis. |
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