Study On Differential Expression Genes Between Cytoplasmic-Nuclear Male-Sterile Line Njcms2a And Maintainer Line Njcms2B Of Soybean

Gene chip technology is an effective approach to study the differential gene expression of different varieties and treatments, which not only can reflect the interaction of each gene, but also can reflect the transcription regulation level of genes under different conditions. Thus, genetic information of the common expressed genes obtained through genome transcription efficiency can facilitate the screening of target genes. In this study, the differential expression genes between soybean cytoplasmic-nuclear male-sterile line NJCMS2A and its maintainer NJCMS2B were investigated using gene chip technology to find out the soybean male-sterile genes and provide basis for creating new soybean male-sterile materials by using genetic engineering. The main results obtained are as follows:1. cDNA microarray was carried out on soybean cytoplasmic-nuclear male-sterile line NJCMS1A and its maintainer line NJCMS1B to obtain their gene expression situation.2176genes with more than2-fold differential expression between NJCMS2A and NJCMS2B, of which1631genes were up-regulated differential expression genes and545genes were down-regulated differential expression genes. Function analysis was carried out using GO (Gene Ontology) classification technique on the174genes with more than2.5-fold differential expression (P<0.01). It was found that these differential expression genes were primarily related to catalytic activity, material transport, metabolism, biological regulation, cell proliferation, and cell construction. EST sequences of the differential expression genes were identified according to the gene ID, which were analyzed comparatively. Some of these174genes were considered to be related to soybean male sterility, including genes related to ATP, polygalacturonase, and so on.2. The genes Gmatp9were cloned from the NJCMS2A and NJCMS2B, respectively. Sequence comparison analysis showed that there were2different bases between the sequences of genes Gmatp9from NJCMS2A and NJCMS2B. The fluorescence quantitative PCR analysis showed that the gene expression of Gmatp9amount in NJCMS2A was significantly higher than that of NJCMS2B, which was consistent with the results of gene chip analysis. Thus, it was speculated that gene Gmatp9may be associated with the male sterility of NJCMS2A.3. The study on Arabidopsis thaliana genetic transformation of gene Gmatp9was carried out. Transgenic Arabidopsis plants of T3generation were obtained through molecular identification. Pollen viability test results showed that pollen viability of Arabidopsis plants with the transgenic Gmatp9decreased. Further research was needed to found out the relationship of gene Gmatp9and male sterility of soybean.