Study On Differential Expression Genes Between Cytoplasmic-Nuclear Male-Sterile Line NJCMS1A And Maintainer Line Njcmsib Of Soybean

Taking the advantage of heterosis is an effective way to improve yield for crop. The genetic research of fertility restoration of cytoplasmic-nuclear male sterility has significant value for the utilization of heterosis. Gene chip technology is an effective way to study the differential expression genes and helps to select the target genes. In this paper, gene chip technology was used to screen the differential expression genes between soybean cytoplasmic-nuclear male-sterile line NJCMS1A and its maintainer NJCMS1B and search the soybean male sterile gene, aimed at providing foundation for research on soybean male sterile mechanism. The main results obtained are as follows:1. Soybean cytoplasmic-nuclear male-sterile line NJCMS1A and its maintainer line NJCMS1B were selected as the material, and then gene chip technology was carried out on them to obtain their bud gene expression profiles.985genes which had significant differences in the expression amount were screened, which429genes were up-regulated differential expression genes and556genes were down-regulated differential expression genes. Function analysis was carried out using GO (Gene Ontology) classification technique on the genes with more than2-fold differential expression. It was found that these differential expression genes were primarily related to protein metabolism, signal transduction, transport, energy metabolism, nucleic acid metabolism and regulation of gene expression, which may be related to the soybean male sterility. Two genes Gmatp6and GmSYP were selected for the further study.2. The whole cDNA of Gmatp6gene was cloned from soybean cytoplasmic-nuclear male-sterile line NJCMS1A. Multiple sequence comparison analysis showed that gene Gmatp6had high homology with gene atp6of other species and a common conserved domain, which indicated that gene Gmatp6belonged to the family of synthase ATP. A plant expression vector transformation Arabidopsis thaliana was constructed to conduct the preliminary functional analysis.3. ATP6was used as the bait protein, gene Gmatp6and gene MADS-box of related to blossom were co-transferred by the yeast two-hybrid technology to verify their interaction. The results showed that there was no interaction between them in the obtained positive clones.4. Gene GmSYP which may be related to male sterility was cloned from NJCMSIB. This gene did not express in the sterile line NJCMS1A, but expressed in the maintainer line NJCMS IB. The bioinformatics analysis showed that gene GmSYP was involved in apoptosis (PCD) pathway, so it may be related to male sterility.