| With the rapid development of aquaculture in China, more and more animal diseases which were caused by bacteria or viruses had taken placed. The use,especially the abuse of feed additive drugs and antibiotics, not only reduces the quality of livestock products remaining in livestock and poultry product that is thermo-stable, endangering human health. but also affects the international competitiveness of livestock products.Development of new, green feed additives is an important research direction of feed industry in the21st century, and microbial is the main way to achieve this purpose and the ideal substitute to replace antibiotics. Compared with antibiotics, enzyme producing prebiotics performs more advantages including non-toxic, no-resistance, no residue and no by-effect, and thus gets more and more attention. The exploitment and use of enzyme producing prebiotics caused national attention, and a large number of experiments and practice has proved that its utilization in animal production shows good results.This paper concentrated on the impact of diets containing Bacillus subtilis2.5×108CFU/g; Lactobacilli0.5×108CFU/g as the main component of industrial enzyme producing prebiotics on chicken eggs, broilers, chicken, suckling pig, duck meat, growth performance, immune function, as well as the product quality, and feeding test was carried out by comparing the effect of enzyme producing prebiotics with antibiotics.The abstract was as follows:Experiment1:Impact of enzyme producing prebiotics on production performance of Roman brown chicken and egg hatching effect90025-week-old Lohmann Brown chicken were randomly divided into five treatments, four replicates each, each repeated45. The basal diet was added with oxytetracycline, complex enzymes and enzyme producing prebiotics, to study the effect on the peak of egg production of breeder and post-production performance of laying, egg hatching effect. The results showed:0.2%enzyme producing prebiotics in feed can significantly increase the egg laying rate (P<0.05), compared with the control group, oxytetracycline group, compound enzyme group and0.1%enzyme producing prebiotics group improved by6.42%,1.99%,5.52%and3.34%, respectively and reduced feed efficiency,,but there was no significant difference in egg hatching rate of each group (P>0.05).Experiment2:Effect of enzyme producing probiotics on broiler production performance23,0001-day-old broilers were taken for experiments,and they were divided into experimental and control groups respectively,11500chickens for experimental group,and the rest of11500chickens for the control group.Broilers for control group were fed with the full price of feed material provided by the Yangtze River, Xuzhou plant biotechnology, for the experimental group,1-21days,22-42days,43-slaughter were fed with the basal diet added the full price feed material containing0.2%,0.15%,0.1%enzyme producing prebiotics respectively. Broilers were seperately weighed then executed the feed consumption statistics, analysis of the data on21th and42nd daysThe results showed that:enzyme supplements (0.2%) can significantly improve the performance of broiler production(P<0.05), feed conversion(P<0.05), disease resistance and survival (P<0.05). Feed supplemented with0.15%,0.1%enzyme could also increase the production performance of broiler, feed conversion, disease resistance and survival, however, the difference was not significant.(P>0.05).Experiment3:Effects of enzyme producing probiotics on growth performance, egg laying rate, egg weight and egg qualityThe experiment was divided into two groups(A and B), focusing on the effect of enzyme producing prebiotics for egg and producing chicken on average daily gain, egg production, egg weight, egg production and egg quality. A trial:901-day-old healthy Hy-Line Brown laying hens were randomly divided into six groups,15for each group, group6th was used for control. The chickens were fed with the basal diet added with0.1%enzyme producing prebiotics at different developmental stages in experimental groups, Group1:enzyme producing probiotics were added on1st7th,14th,21th,28th,,35th,42th days, group2nd enzyme producing probiotics were added on the1st,7th,14th,21th,28th days, group3added on the7th,14th days, group4th was added on the first14th,21th,28th,35th42th days, group5th was added in the first28th,35th,42th days and no feed additives were added to the control group feed was provided by the Yangtze River biotechnology Xuzhou. Then measured chicken weight in the first10,21,30,40days of experiment.The results showed that feed added with enzyme producing prebiotics improved significantly average daily gain of function (P<0.05). B trial:80025-week-old Lohmann laying hens were randomly divided into four groups, control group and antibiotic group (which contains bacitracin zinc and sulfuric acid20mg/kg Colistin4mg/kg),0.1%enzyme producing prebiotics group and0.2%enzyme probiotic group. The results showed that laying rate, egg weight and egg production of treatment group by the0.1%enzyme producing prebiotics was better than the that of control group and0.2%enzyme producing prebiotics treatment group, significantly higher than the antibiotic treatment group (p <0.05). The egg laying rate of0.1%enzyme producing probiotics treated group was significantly higher than the one of antibiotic treated group (p<0.05), egg weight of0.1%enzyme producing probiotics treated group was significantly higher antibiotic treated group (P<0.05), the egg production of0.1%enzyme producing probiotics treated group was significantly higher than antibiotic treated group (P<0.05).We also found that Egg laying rate, egg weight and egg production of0.1%enzyme producing prebiotics treatment groups was better than that of the0.2%enzyme producing prebiotics treatment group, indicating that a large number of experimental studies will be needed to determine the amout of beneficial bacteria in poultry feed.Experiment4Effect of enzyme producing probiotics on pigs production performance48triple-hybrid pigs, based on the principle of body weight, similar sex ratio are divided into Ⅰ、Ⅱ、Ⅲthree groups, while Ⅰ was the control group, Ⅰ、Ⅱ、Ⅲgroup for the test group, each16, no duplicate. Male and female in half, separately numbered; the same basic diet and nutrition line was for each group, group Ⅰ were fed on conventional corn-soybean meal diets without adding any enzymes, group Ⅱ were added0.1%Bora Lee to produce enzymes enzyme producing prebiotics in the on the basis of the basal diet for group Ⅰ, and group Ⅲ was added0.1%enzyme the on the basis of the basal diet for group Ⅰ. The results of feeding experiment showed that:feeding on conventional corn-soybean diets average daily gain581g, feed conversion rate2.38; the basal diet added with0.1%enzyme producing prebiotics average daily gain650g, feed conversion rate2.17; the basal diet added with0.1%enzyme average daily gain604g, feed conversion rate2.20. After adding probiotics and enzyme,we found that the average daily gain and feed conversion rate increased, but the difference was not significant (P>0.05). To analyze the economic efficiency on the basis of weight gain and feed costs,overall economic efficiency of feed per kg weight gain cost for groups B, C was3.89yuan,3.95yuan,4.26yuan for the control group,respectively and economic efficiency for group B increased by9.51%.Experiment5:Comparison of effect of enzyme producing prebiotics with antibiotics on feedingThe experiment compared0.3%probiotic with antibiotics on feeding effect.40breeding Tianrong pigs were randomly divided into group A and group B, each pig earmarked, respectively.0.3%enzyme producing probiotics was added in the test group A, antibiotics for the control group B.Feeding trial showed that:the daily gain of experimental group was slightly higher, but the difference was not significant (P>0.05); the daily feed intake of experimental group was slightly higher, but the difference was not significant (P>0.05). The difference of feed conversion rate between experimental and control groups was not significant (P>0.05). But obvious advantages had taken place in the appearance and economic assessment (P<0.05). |
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