Screening And Identification Of Affinity Peptides To S-AD Protein Of Porcine Transmissible Gastroenteritis Virus

Porcine transmissible gastroenteritis virus (TGEV) caused by porcine transmissible gastroenteritis is an highly contagious enteric diaease of swine, which was characterized acute chordapsus, diarrhea and death in piglets. The disease is widespread around the world, Bring great losses to pig industry.Thus, developing an effective vaccine is an important research topic for prevention of TGEV infection, protein (S) is the TGEV surface glycoproteins,whitch induced animal immune system to produce specific neutralizing antibody And can be mediated virus specific receptors on the cell membrane,involved in cell membrane fusion process.This experiment using recombinant plasmidpET30a-S-AD, whitch contains the S-AD (1102bp-1812bp) highly expressed the fusion protein in Escherichia Coli Rosseta (DE3).SDS-PAGE results showed that the expression of protein molecular weight is34KDa. form the results of the Western-Blot, we geted that the protein can reacted with positive serum of TGEV specificity, indicated that the recombinant protein has a very well biological activity.We compouned the peptide with the best affinity, named T. The Sequence is followed:-T-L-N-M-H-L-F-P-F-H-T-G-. We used the method of that detected the TCID50of virus, detected the fluorescent signal by indirect immunofluorescence, calculated the reduction of plaques number to detect the antiviral activity of the synthetic peptides in vitroantiviral activity. Results showed that the way TGEV and T peptides after1h of incubation at37℃, joined into ST cells had a high effect to inhibit the infectivity of TGEV.The current study may be helpful to design preventive strategies to TGEV infection based on phage display techniques.