Study On Application Of Lactobacillus Plantaru，Bacillus Subtilis And Compiex-probiotics In Diets For Weaned Piglets And Calves

In these series of research, two different strains which were isolated from soil and applied in weanedpiglets and calves. The researches included effects of probiotics on growth performance, digestibility,immune organ indexes, and gastrointestinal pH of weaned piglets and effects of probiotics on growthperformance, digestibility, FCI and resistance of stress of calves. The present research consists of twoexperiments which were listed as follow. The research was finished inExperiment1: Effects of probiotics on growth performance, digestibility, immune organ indexes, andgastrointestinal pH of weaned pigletsOne hundred and twenty-eight weaned piglets were allocated into4groups balanced for sex, with4replicate and8pigs in each replicate. The duration of the experiment was35days. Piglets in4groups werefed with the following solutions: Piglets of CT were fed with basal diet as control, LB were fed with basaldiet containing Lactobacillus plantarum GF103（8.6×109CFU/pig/d）, BS were fed with basal dietcontaining Bacillus subtilis （2.0×10⁸CFU/pig/d） and LBS were fed with basal diet containing Lactobacillusplantarum GF103（4.3×109CFU/pig/d） and Bacillus subtilis （1.0×10⁸CFU/pig/d）. During the first twoweeks of the trial, the piglets supplemented with probiotics had better feed conversion ratio （FCR） whencompared with that of control （P<0.05）. Digestibility of LBS was improved in pigs fed with probiotics,although there was no significant difference. Liver organ indexes of LBS was significantly increased（P<0.05）, simultaneously, the pH of stomach and duodenum was significantly decreased than CT （P<0.05）.It is concluded that growth performance, feed cost, immunity, and gut environment of weaned piglets canbe improved when probiotics were supplemented in pigs’ diets. However, the effects weakened with timepassing by.Experiment2: Effects of probiotics on growth performance, digestibility, FCI and resistance ofstress of calves. Twenty-four newborn Holstein calves were randomly divided into3groups with4males and4femaleseach. A basal diet without antibiotics or probiotics was used as control, and the other two group were fedthe control diet supplemented with Lactobacillus plantarum GF103(1.7×10¹⁰CFU/calve/d) as LB group orwith complexes of Lactobacillus plantarum GF103(1.7×10¹⁰CFU/calve/d) and Bacillus subtilis B27（2.0×10⁸CFU/calve/d） as LBS group. The trial lasted12weeks. The results showed that there were nosignificant difference on growth performance （P>0.05）, but feed conversion ratio was improved in calvesfed with probiotics during8-10weeks （P<0.05）. The FCI of calves supplemented with probiotics wasimproved but not significantly. Digestibility of P, N, and ABV of calves supplemented with probiotics wereincreased significantly （P<0.05）. The T-lymphocyte transformation rate of LBS group was increased andthe cortisol concentration of LBS group was decreased significantly compared with CT. In conclusion,dietary probiotics supplementation increased daily gain, decreased F/G during early life of calves;simultaneously, resistance of stress during weaning.