| Very-long-chain fatty acids (VLCFAs) contain18or more carbon atoms,including saturated fatty acids and unsaturated fatty acids. VLCFAs are not only an important biological membrane component, but also play a key role in cell growth and development. It was proved that VLCFAs exogenously applied in ovule culture medium could promote cotton fiber elongation. GhKCSs code3-ketoacyl-CoA synthase (KCS) which are the key enzymes of very long chain fatty acid synthesis. GhKCSs were introduced into Gossypium hirsutum L. cv.’zhongmiansuo24’by Agrobacterium-mediated transformation to verify their function in progress of cotton fiber elongation. The main results are as follows:1. We got six positive transformant by Agrobacterium-mediated transformation.2. Over-expression of GhKCS6could improve the fiber length and fiber strength by comparing to the control treatment, the other fiber qualities for example micronaire value and fiber elongation didn’t have a significant difference.3. GhKCS6could promote the fiber elongation until the expression reached certain level. By analyzing the expression level of GhKCS6in different transformant, the result showed that not all the positive transformant could promote the fiber elongation. Among three transformant, the fiber length of the transforman in which the gene had the highest expression level had a significant difference compared to the control, the other two transformant couldn’t promote the fiber elongation but could improve fiber strength.4. GhKCS6regulated the fiber elongation by activating expression of a series of genes. Compared to the control, the expression of a series of genes relate to fiber elongation had a remark change in the transgenic plant. |
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