Investigation Of Intestinal Patasites Infection And Identification On Species Of Cyclospora And Cryptosporidium From The Captive Wild Animals In Shaanxi Province

Wild animals are human’s precious wealth, but also the main reservoir of zoonoticpathogens. With the development of eco-tourism and transport making close contact betweenhumans and wildlife, challenges were brought to human health while making people morehappy. Therefore, it is necessary to conduct a comprehensive survey and in-depth study forintestinal parasites of wild animals. In this study, the survey on the species of intestinalparasites from captive wild animals within Qinling Mountains area in Shaanxi Province wasconducted and the species and subtype of Cyclospora and Cryptosporidium isolates obtainedwere identified respectively.1．In order to fully understand the intestinal parasites infection of the main wild animalsin Qinling Mountains, the Saturated sucrose flotation and Lugo type liquid iodine stainingmethods were used to detect965stool samples from51species of animals in Shaanxi RareWildlife Rescue Breeding Research Center and Qinling Wildlife Park. Parasites belonging to8genera were detected in42animal species, including Coccidia, Cyclospora, Cryptosporidium,Amoeba, Strongyloids, Trichuris, Capillaria and Ascarid. Results also showed that Characteristics of theintestinal parasites infection in captive wild animal were mixed infections and unbalancedinfection nature. In addition, there is a certain relationship between gender and parasite infection ratein takins, the infection rate were higher from male than female with significant difference.2．For identification of Cyclospora sp. in golden monkey (Rhinopithecus) using18SrDNA, genomic DNA of two Cyclospora isolates were amplified by Nested PCR and theamplicons were sequenced with the length of500bp approximately. Phylogenetic analysisshowed that the Cyclospora isolate from golden monkeys was located in the branch ofCyclospora and sistered to C. colobi.3．The species of Cryptosporidium isolates from takins and camels were identified basedon the18S rDNA, indicating that Cryptosporidium spp. from takins were C. andersoni and C.parvum, and the ones from camels were C. andersoni. There are two different C. andersoniisolates from takins, with96.1%of sequence identity. The similarity between takin-derived C.andersoni isolates LN1, LN2, camel-derived C. andersoni isolates LT4and C. andersoni （JX515549）in GenBank is100%,96.1%,100%respectively.4．Subtyping C. parvum from takins based on the GP60gene loci, a new subtype wasfound and named as IIaA11. The subtype of C. andersoni from takins was identified as A1based on the MS16genes and the ones from camel were identified as A2and A1, respectively,based on the MS2and MS16genes. At the MS2loci, the similarity between C. andersoniisolates from camels and A1(HM565078) in GenBank was99.8%. At the MS16loci, thesimilarity between all C. andersoni isolates and C. andersoni（HM565086）in GenBank was100%.