Proteomic Analysis On Different Genotype Broilers Liver

Socio-economic development and improvement of living standards of people make them pay moreattention to safer, more delicious and healthy animal product. In the negative background of fast growbroiler, the consumer prefer local breeds for its meet quality.As the key metabolic organ in broiler, liver plays an important role involved protein, carbohydrateand fat metabolism at all development stages. Therefore, liver can be used as suitable target organ formetabolism researching.what’s more, liver proteome has a positive understanging of metabolic changes.We investigated the Protein Profiling of AA male broiler and Dagu rooster liver at14days,28daysand42days using2-DE and differential expressions by masss Pectrometry.Comparing two breeds ofprotein profiling at different stages, we identify differentially expressed protein between two breeds atdifferent stages and classify the Proteins, carry out funetional enriehrnent, Proteins interaction net andcluster analysis. It’s Aiming to illuminate the molecular mechanism of AA broiler and Dagu chickengrowth and metabolic regulation. Lay the foundation for the promotion of broiler breeding.The results showed that the detected spot number on14days,28days and42days of the AA broilerwere364,442and475, respectively. Whereas, the detected spot number on14days,28days and42days of the Dagu chicken were363,435,400, respectively. The expression protein of AA broiler liverwas increased gradually, peaked at42days, however, Dagu chicken reached a peak at at28days of age,which indicating trends of gene expression at different stages were different between two breeds.From the expression protein of AA broiler and Dagu chicken at all stages, AA broiler expressedprotein306and Dagu chicken expressed protein278.249of these proteins were expressed at all stages,indicating that these proteins is needed for chicken liver development, and regulating the developmentalprocess by similar expressional pattern.Comparing differential protein expression in two breeds at all stages, we divided them into ninecategories by biological processes. According to functional classification ofthe identified proteins，33proteins were related to the metabolism of carbohydrates metabolism and energy production(22.6%),32proteins belonged to protein and amino acid metabolism(21.92%),14protein were related to response tostress and antioxidant(9.59%),14proteins belonged to cell structure(9.59%),12protein were related tonucleotide metabolism(8.22%),12proteins belonged to fatty acid metabolism(8.22%),7protein wererelated to protein folding(4.79%),5proteins belonged to transporter(3.42%),and17protein were relatedto unknown function(11.64%).Analysis of protein interaction prediction showed that protein involved in carbohydrate metabolismand energy production ME1, MDH1, GLDC, protein involved in protein and amino acid metabolismGMPS, PRPS2, GART, protein involved in fatty acid metabolism L-FABP, ACADL, ECI1were keyprotein in chicken hepatic metabolism. Protein involved in response to stress and antioxidant, TXN、PRDX6and PRDX1were the key protein. Protein involved in cell structure TPM1and DES were thekey protein. Protein involved in protein folding HSPs, HSPE1and ST13were the key protein. Abovethese proteins were as the candidate protein between two breeds.