The Technology Of Rape Bee Pollen In Acidic Environment

Bee pollen is the male reproductive cells, contains various nutrients required forplant breeding offspring, known as the "micro nutrition library".from the bee pollenidentified the flavonoids, polysaccharides, amino acids, protein, vitamins, traceelements, active enzyme, lipid, nucleic acid and other chemical composition, and onits pharmacological effects are also studied.Due to pollen outside the cell membrane with a thick layer of cell wall, is notconducive to full release active ingredients in the cell. Cell wall-breaking techniqueis to put the plant cell wall, make the effective component inside the cells in thedamaged cases completely released. Now common pollen wall-breaking method are:physical method, chemical method and biological method. Physical wall-breakingmethod, including mechanical wall-breaking method, the temperature differencemethod, water immersion stress wall-breaking method, puffing wall-breakingmethod, liquid nitrogen quenching cooling method, ultrasonic wall-breaking method;Chemical wall-breaking method has wall-breaking method, organic solventextraction wall-breaking method, trigger wall-breaking method; Biochemicalwall-breaking methods including bee bread wall-breaking method, mixed enzymewall-breaking method, microorganism fermentation wall-breaking method.In this paper, through study of rape bee pollen broken walls in the acidenvironment, determined the optimum solid liquid ratio, temperature, acidity, time,and the kinds of acid; And through the double beam uv spectrophotometerdetermination not to break the wall and the broken wall of free amino acid content inrape bee pollen, with722type spectrophotometer determination not to break the walland the broken wall of the flavonoids content in rape bee pollen, with azotometerKDN-type,876with liquid and semi automatic potentiometric titration instrumentmeasuring the protein content in rape bee pollen, with air-acetylene flame atomicabsorption spectrometry is not broken and the broken wall of rape bee pollen contentof mineral elements in the samples, and for the first time using thermogravimetricanalysis on the wall and wall of rape bee pollen unsolved thermal analysis was carried out. To rape bee pollen under acidic conditions to break the wall of theindustrial production conditions.