Study On The Pharmacokinetics Of Cefquinome Sulfate In Goats

The establishment of detection method and pharmacokinetics of cefquinome sulfate were investigated respectively after intravenous injection and intramuscular injection at the dose of2mg·kg-l b·w·in healthy goats.First time,the detection method of plasma concentration of cefquinome sulfate in goats was established by HPLC. The experiment results showed the method was line with the relevant requirement of the pharmacokinetic studies because of its good reproducibility, accuracy and precision. The main indicator parameters were as follows:the limit of detection was0.05μg/mL, limit of quantification0.μg/mL, recovery was91.11to97.20%, the coefficient of variation was0.425%to2.269%, the day coefficients of variation was0.357%to4.652%.6goats were randomly divided into two groups(Ⅰ,Ⅱ) for test respectively after intravenous injection and intramuscular injection. Blood samples were collected at different intervals after administration of the two methods. The cefquinome sulfate concentration in plasma of the two routs of administration were determined by high performance liquid chromatography (HPLC). The concentration-time data of cefquinome sulfate in plasma after intravenous injection and intramuscular injection was respectively well fitted for two-compartment model with no absorption and a two-compartment open model with first-order absorption by3P97computer program. The mathematical equations were respectively C=3.58e-4.392T+0.65e-0.2278Tand C=3.949e-0.251T+0.705e-0.094T-4.654e-0.381T.The main pharmacokinetic parameters of the two routes of administration in goats were as follows(1)intravenous administration:t1/2α=0.158±0.012h, t1/2β=3.075±0.298h, AUC=34.289±0.113mg/L*h, V1=0.609±0.086L/kg,CL=1.904±2.501L/h/kg;(2)intramuscular administration:ti/2α=2.757±0.457h,t i/2p=7.341±0.841h, AUC=27.269±0.352mg/L*h V1=0.903±0.547L/kg,Tmax=4±0.649h, CL=0.273±1.547L/h/kg, Cmax=1.142±0.547μg/mL, F=79.5%The result showed that cefquinome sulfate by intramuscular administration with 2mg.kg-1.bw was slowly absorbed in goats with low peak drug concentration and long peak drug time, but the drug has relatively long T max, high maintained plasma levels and long effective concentration duration and high absolute bioavailability. Tissue Residues of cefquinome sulfate in goats would be further researched.