The Research Of SSR Loci In Astragalus

This experiment adopts the high salt CTAB method to extract the genomic DNA of Mongolian Astragalus membranaceus, Astragalus membranaceus Bge and Astragalus chinensis.Twenty-eight soybean SSR primers are used.I also design two pairs of SSR primers in according of Astragalus EST sequence at the same time.This primers are used by PCR amplification in this experiment..The results show that seven pairs of primers amplify out of the SSR alleles from the genomic DNA of Astragalus. There are five primers which can be used for identification of authenticity Astragalus and falsify. Because the SSR amplification bands are clear and easy to distinguish. The last two primers can not be used. The reason is that the SSR amplification bands which have low discrete degree and not easy to distinguish. Astragalus genome information is limited, but we can borrow the same family plant of soybean SSR primers that can avoid the construction of gene library.The seven primers produce different length and the number of alleles of different SSR sequence which are their differences. Material similarity coefficient which shows the similarity of primer amplification can reflect the different between the authentic Astragalus and falsify.The experiment results show that seven of thirty primers have polymorphism in PCR-amplified. It will be as a reference for the study of Astragalus SSR molecular basis. It also can be used to identify authenticity Astragalus and falsify, It prove that the same family plant SSR primers for PCR is feasible.