| Cannabis Sativa L, annual herbaceous plants of the genus Cannabis, with a long history of culturing, is an ancient bast fibers crop. In recent years, due to the lower production of cannabis cultivation, as well as competition from other fiber plants, the planting area and production of cannabis is decreased. Therefore, to effectively improve the production of industrial hemp and to further improve the quality of industrial hemp fiber is undoubtedly critical to the overall industrial development of cannabis. It has been reported that endophytic fungi has the potential to improve the nutrient absorption and utilization efficiency of many other host plants. Hence, it is worth to be trying to improving the production and quality of cannabis. Some of the endophytic fungi, which exist in fiber plant, might be potential source of agent for fiber growth promoting. At present, there is no studies were reported on endophytic fungi in fiber plants. In this study, we isolated endophytic fungi from different tissues of the same and different cannabis varieties in different sample sites. Based on morphology and ITS sequence, the isolated endophytic fungi were identified and the diversity and distribution characteristics of endophytic fungi in different strains of cannabis were analyzed. From the isolated endophytic fungi strains,6strains of fungi were chosen to conduct the field re-inoculation experiment, studying the influence on the physiological activity and agronomic traits on cannabis. The preliminary results were listed below:1. In total531strains of cultivated endophytic fungi were isolated from stem and leaf samples of industrial hemp (Yunnan hemp1). Among,138strains were isolated from stem and393strains from leaf. All these endophytes belong to29genera (22genera from stem;24genera from leave), of which18genera were found both in stem and leaf. Colletotrichum was the dominant genus with dominance index up to40.11%.2. The isolation rate of endophytic fungi in stem was evidently lower than that in leaf. Overall, the highest isolation rate was found in the samples from the basal part of hemp plants. The diversity index of endophytic fungi in hemp from the four sampling sites varied from1.84to2.46. The similarity coefficients were relatively high among all sampling sites, but lower between the stem and the leaf from the same site.3. In total376strains of foliar endophytic fungi were isolated from different cultivars of Cannabis sativa L.(of which71strains from cultivar of Yunma No.3,107strains from Yunma No.4,95strains from Yunma No.5and103strains from Yunwan No.6), and identified belonging to20genera, of which6genera of fungi were found in all cultivars. Colletotrichum was the dominant genus, and the dominance index was more than50%in all the detected4hemp cultivars. Isolation rate of endophytic fungi from hemp tissues ranged from0.47to0.71, with an average was0.63.4. Inoculation of Chaetomium sp. and Fusarium sp. increased the activity(P<0.05)of SOD and POD. Dry weight and thickness of hemp fiber were improved when inoculated with Plectosphaerella sp., Chaetomium sp., Nigrospora sp., Fusarium sp. and Graphium sp. The inoculation of Chaetomium sp. can significantlyincrease the length of internodes, dry weight and thickness of hemp fiber (P<0.05). |
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