Methylation Sensitive Amplification Polymorphism(MSAP) Analysis Of Mainly Cultivated Oyster

The yield of edible mushroom of China is the largest in the world and the mushroom industry is the biggest industry after food, oil, fruit and vegetables in China. The yield of oyster mushroom(Pleurotus ostreatus) occupies the first place among the all artificial cultivatied edible mushroom species. With delicious flavour and amount of nutrition, oyster mushroom have great food and medicinal value. However, the growth and development of oyster mushroom possess complicated mechanisms and can be influenced easily by environmental factors. And in the aspects of breeding, strains preservation and cultivation, many problems remain to be solved.As the key point of epigenetics, DNA methylation is involved in growth, development, senescence, death and resistance of stress, almost the all metabolism activities of organisms, playing an important role in the regulation of these activities.Using the method of Methylation Sensitive Amplification Polymorphism(MSAP), the research analysed the levels and modes of genome DNA methylation of nine state-certified oyster mushroom strains, aiming at studying the levels and models of genome DNA methylation of oyster mushroom at natural state, and the effect of temperature stress on the levels and models of genome DNA methylation. At the same time, the research got the best experiment condition and parameters of MSAP used in the study of genome DNA methylation of oyster mushroom, paving the road for the study of the impact of DNA methylation on the growth, development and resistance of stress of edible mushroom. The main results are as follows:1. The research got the best experiment condition and parameters of MSAP used in the study of genome DNA methylation of oyster mushroom, paving the road of DNA methylation study of edible mushroom.2. The research got the levels and modes of genome DNA methylation of the nine oyster mushroom strains, finding that the levels of DNA methylation range from1.9%to17.2%, and the mean DNA methylation proportion is8.9%. It was evident that DNA methylation exist generally in the genomes of the nine materials and there were significant differences between them. In addition, double-chain methylation was the main mode. 3. Taking4℃and40℃for24h as the temperature stress treatment condition, and25℃as the control group, the research studied the effect of temperature stress on DNA methylation of the materials. The result showed that temperature stress can influence the level and mode of DNA methylation, but whether the changes of DNA methylation resulted from temperature stress play roles in the adaptation to environment of materials needs to be further studied.