Study On Rapid Propagation And Regeneration Of Atractylodes Lancea Seedlings

The effects of different of plant growth regulators (PGRs) on inducing adventitious buds and the secondary culture from different explants of shoots, stem segements, leaves, petioles, root and buds in Atractylodes lancea after the selection of the better sampling stage and suitable sterilization time, and the calli induced from leaves, petioles and shoots in A. lancea and redifferentiation system were mainly studied in this paper. The purpose of the research is to establish the system for rapid propagation and the process for redifferentiation from the calli of Atractylodes lancea and the better protocol for rooting, harding and transplanting. The main results are as followed:1. The bud regeneration time and rate were associated with the types of explants of Atractylodes lancea. The frequency of different explants for bud regeneration rate followed the order:stem tip>stem>leaf>petiole>rhizame bud>rhizame; the sequence time for bud regeneration needed for culture was that stem tip>stem>rhizame bud> leaf>petiole>rhizame. The shoot and stem are more suitable for buds regeneration in rapid propagation of seedlings in Atractylodes lancea based on the redifferentiation rate and time.2. The suitable concetration of6-BA and NAA in MS medium for inducing bud regeneration depended on the type of explants. The bud regeneration rate induced from shoots almost reached100%on the MS medium with the combination of6-BA with higher concetration (1.0～3.0mg·L-1) and NAA with lower concetration (0.2～1.0mg·L-1). MS contained1.0mg·L-16-BA and0.5mg·L-1NAA was better for the bud regeneration from the stem, and MS combined with2.0mg·L-16-BA and1.0mg·L-1NAA was more effective for the bud induction from the explants of leaves and petiole; but the regenerated buds induced from the explant of rhizame bud growed very slowly, and no regeneration of bud from the explant of rhizame in A. lancea. MS contained0.5mg·L-16-BA is optimum for the successive proliferation of seedlings.3. Combination of PGRs showed different effect on dedifferentiation of different explants for calli induction. MS with0.1mg·L·16-BA and0.2mg·L-1NAA was proper for calli induced from leaves and petioles, and MS with addition of0.2mg·L-12,4-D and0.2mg·L-1KT was better from the shoots. MS contained1.0mg·L-16-BA and0.1mg·L-1NAA was more effective for the redifferentiation. 4. The optimal medium for rooting of seedlings in Atractylodes lancea was MS contained with0.5mg·L-1NAA.