Researches On Tissue Culture And Rapid Propagation Of Atractylodes Lancea In Baokang

Atractylodes lancea,a perennial herb belonging to Composite,Atractylodes,is a kind of common medicine plant with some pharmaceutical effects.Baokang County,Hubei Province has aboundant wild Atractylodes lance resources.In order to promote the protection and exploitation of the resources,researches on tisuue culture and rapid propagation of Atractylodes lancea from Baokang were conducted in this paper.Sterile systems were firstly established with rhizome lateral buds,stem segements with axillary bud,leaves,petioles and internode stems as explants;then researches on the optimum culture medium for axiallary buds sprouting induction,shoots proliferation and roots induction were carried out and the rapid propagation was estabolished;thirdly,the media formulation and culture condition for organogenesis was researched.Main results were as follows:(1)Explants sterilization:collected the rhizome lateral buds in October and November 2012,cleaned away the rotten barks,immersed in 0.1%bavistin for 10min and in detergent solution for 5min,then flushed 1-2h in running water.Then,transferred the explants into laminar flow,sterilized them with 70%alcohol for 30s and with aqueous solution of mercuric chloride(HgCl2)for 6min,and finally washed the explants with sterilized water for 5 times.In March 2013,collected explants such as stem segments with axillary buds,internode stem segments,leaves and petioles;wipe away the surface pubescence with gauze,then sterilized the explants with the same treatments with the rhizome lateral buds except without 0.1%bavistin treating for 10min.Results showed that it was best to sterilze the stem segments with axillary buds,internode stem segments and leaves for 6min and to treate petioles for 7min.(2)Compared with DKW medium,MS medium was more suitable for axillary bud initiation culture.The best medium for axillary buds initiation culture was MS+6-BA 1.5 mg/L + NAA 0.4 mg/L.(3)The best shoot proliferation medium was MS + 6-BA 2.0 mg/L + NAA 0.6 mg/L,the proliferation times reached 3.77 and valid shoot rate was 92.05%.(4)The best medium for rooting was MS + NAA 0.3 mg/L or MS + IBA 0.3 mg/L,the rooting rate was 100%.(5)The orthogonal experiment for calli induction showed that leaves and petioles were the suitable explants,the best culture medium was MS+2,4-D 1.0 mg/L + NAA 0.2 mg/L,100%explants produced calli with high quantity and good quality.But no shoots differentiated from the calli.(6)In the direct organogenesis experiments with leaf and petiole explants,the explants got browning gradually and died at last.Therefore it is necessary to do some further researches.