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Morphology Study On The Development Of The Silkie Intestinal Mucosal Immune System

Posted on:2014-11-26Degree:MasterType:Thesis
Country:ChinaCandidate:L WangFull Text:PDF
GTID:2253330401968260Subject:Basic veterinary science
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silky fowls which have the high quality of protein are always choosen for nutritional necessitis. More content of8different kinds of microelement and more vitamins than the plant. The demand of silky fowls is increased and the premise of the cultivation of silky fowls is industrial integrity and a great quantity of findings. Thus, the research about the silky fowl’s productivity, feeding, management and disease prevention is indispensable.First,1-9weeks old silky fowls are used for this study for investigate the morphological structure of small intestine, The reasult shows that duodenum and jejunum have the same regularity of development and ileum shows the different regularity. This study provided the basis for cultivation.Second, the PAS staining showed that the developmental characteristic of goblet cells is similar with development of small intestinal morphology. Moreover, jejunum is the main functional site of intestinal mucosal immune system.Third, the azurophilic special staining was used in this study. The result shows that5-7weeks is the critical period about the change of immunifaction’s site of plasmocytes:they are educe the function of immunologic barrier in ileum before5-7weeks, on the contrary, after the5-7week is in duodenum.Forth, the paraffin section and toluidine blue special staining to investigate the distributional characteristics and the functional stage of mast cells which play an important role in the immunologic barrier. The reasult indicate that the most important functional stage of mast cells in immunologic barrier is5-7weeks, moreover, the site where mast cells play a role in immunologic barrier is1-7weeks’ileum,7-9weeks is jejunum.Fifth, The SABC immunohistochemistry method is used to investigate the cytokine’s distributional characteristics during development. The result shows that IL-21is mainly in the duodenum. Therefore, the most important functional stage of mucosal immune system that IL-21played a role in is5-7weeks and the mainly site is in duodenum.
Keywords/Search Tags:Taihe silkie, small intestine, mucosal immune system, morphosis, developmental characteristics
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