The Distribution And Changing Of IL-21and Mast Cells In Silkie Immune Organs Development

The silkie central immune organs containing thymus and bursa, while peripheral lymphoid organs including spleen and the lymphoid tissue of enteron, respiratory tract and genitourinary tract. Immune cell differentiation and maturity take place in the central lymphoid organ, the peripheral lymphoid organ is the place where the ripe immune cell settle down, proliferate and develop the immune response. Along with the increase of ages, the immune organ will become degeneration that with different levels, so, the immunity of body is declining, which induce the occurrence of disease.As a very significant cytokine, IL-21plays a very important role in the process of cellular immunity and humoral immunity. Mast cells are the basal cell of anti-infections immunity, which have the function of phagocytize and clear bacteria. In the process of occurrence of diseases, the action of mast cells is very important. The study of IL-21and mast cells is mainly focus on human and mammals, while in the silkies’immune organ, its distribution and development changing was not reported.Therefore, silkie was chosen as experimental animal in the study, in different days (7,14,21,35,49,63,112days), get their thymus, bursa and spleen. We use the technique of paraffin section, staining, immunohistochemistry and toluidine blue staining to study IL-21and mast cells’distribution and development changing in silkies’ immune organs, and histologic structure and development changing. The main research shows that:1The histological structure characteristics and development changing of silkie immune organsTo study silkie immune organs histological structure characteristics and development changing, the paraffin section technique and HE staining technique were used in the study. The result shows that, the silkie thymus cortical dyeing is deep, violet, the silkie thymus medulla dyeing is shallow, pale pink. At7days, the boundary of thymus cortex and medulla is not obvious, after14days, the boundary is obvious. The capsule is the most thick at63days, it is the most thin at7days. The silkie bursa is made up of mucous membrane, submucosa, muscular layer, tunica adventitia. At the49days, there are many goblet cells in the mucous membrane. Before of14days, the boundary of bursa cortex and medulla is not obvious, after21days, the boundary is obvious. The bursa medulla has paged phenomenon at49days and63days. The capsule is the most thick at63days, it is the most thin at7days. The silkie spleen marginal zone and red pulp are pale pink, white pulp is violet. The capsule is the most thick at49days, it is the most thin at7days. The splenic corpuscle diameter is the largest at35days. It is the smallest at7days.2The distribution characteristics and development changing of Mast cells in the silkie immune organsIn order to investigate the developmental changes of mast cells in the immune organs of silkie, the study had taken the toluidine blue staining technique to study the distribution characteristics and development changing, cell morphology and developmental changes of mast cells in silkie immune organs. The result shows that a large number of mast cells distributed in silkie thymus, which mainly distribute in medulla. There are many mast cells distributed around the thymic corpuscles, less mast cells distributed in the cortex and the junction of cortex and medulla, the interstitial portion of the adjacent thymic lobule has the least mast cells. The cells are mostly round or oval, blue-violet, consistent size, unconspicuous nuclei. There are some differences about the quantity of mast cells in different ages of silkie. Mast cells in silkie bursa are mainly distributed in the mucosal epithelium and the epithelial thicket, there are less mast cells distributed in the cortex, these cells are mostly round or oval, purple, slight differences in size and unconspicuous nuclei. Mast cells are mainly distributed in the red pulp of the silkie spleen, while the white pulp is less distributed. Mast cells are blue-purple, consistent size, round or oval. A small amount of mast cells present irregular types, smaller diameter, unconspicuous nuclei, some cells periphery have apophysis.3The distribution characteristics and development changing of IL-21in the silkie immune organsWe use the immunohistochemical technique to study the distribution characters and development changing of the IL-21in silkie immune organs, aiming to explore the role of IL-21in the growth and development of silkie and to explore the role of IL-21on the immune system of silkie. This study provide a theoretical basis for silkie breeding and disease control and prevention.The result showed that IL-21is mainly located in the medulla, the cortex is less. There are more positive cells distributed around the thymus bodies, which are brown, round, similar diameter and the nucleus of these cells is not obvious.112days of age, the nucleus is clearly visible. There is some differences about the positive signal strength in different days of silkie. IL-21is mainly distributed in the white pulp of silkie spleen, while the red pulp is less, positive signal also distribute in the marginal zone and near the ellipsoid. There are more positive signal distributed around the splenic nodule. Positive cells are nearly circular, smaller diameter, claybank and the nuclei are not obvious. IL-21are mainly distributed in the mucosal epithelium, epithelial plexus and medulla in the bursa of silkie, while there are less distributed in the cortex and lamina propria. Positive cells are brown, large diameter differences and the nuclei are not obvious.