The Expression Of Ghrelin In Testes At Different Stages Of The Postnatal Mice

In order to study the expression pattern of Ghrelin in testes at different stages ofpostnatal mice, testes from mice of1～16weeks old were obtained as experimentalmaterial.The histological characteristics observed by paraffin section-H.E. staining, and studythe distribution of Ghrelin by immunohistochemical method.The results as follows:1. Spermatogenic cells in seminiferous tubule proliferated and developed gradually as theage of mouse，and became more and more intensive. There were only spermatogonium andthe sertoli cells in seminiferous epithelium at1～2weeks; meanwhile, sertoli cells betweenspermatogenic cells became typical and easy to identify, nucleoplasmic ratio of sertoli cellsdecreased with weeks increased. Interstitial tissue of testis thining with increased pipediameter, shape of leydig cells mature became mature gradually and eosinophilic increasedgradually. We divided seminiferous epithelium cycle into12spermatogenic stages accordingto the histological characteristics.2.Positive reaction was observed mainly in seminiferous epithelium and leydig cells, andshows regula expression: Ghrelin in testicular capsule were clearly, no espression inseminiferous epithelium and low expression in leydig cells at1～2weeks. Positive resultsdistributed in spermatogenic cells and spermatocyte, increased in leydig cells at4weeks.Positive expression Ghrelin are mainly distributed in leydig cell cytoplasm and sertoli cells,sperm cells have a weak positive reaction. After8th weeks, ghrelin mainly expressed inleydig cells and sertoli cells, the relative transcript level increased with the growth anddevelopment of postnatal mice. increased obviously in4th weeks and strongest in16th weeks.In sum, this results showed that Ghrelin play an important role in the process ofspermatogenesis, and has potential influences on the synthesis and secretion of steroidhormone。...