Study On Tissue Culture And Gene Transformation Of Torenia Fournieri

We did carried out a series of research about Torenia fournieri L for tissue culture andgenetic transformation studies in this paper. For one, we changed the amount of nitrogen inthe culture elements in order to make an earlier flowering. For two, suspension culture systemwas established and plant regeneration was inducted. For three, the method of genetransformation was used to transform Lc and the PAP1gene into Torenia and increases itsexpressing level of anthocyanins, and finally change plant color.In the experimental induction of flowering project,1/3MS(Murashige and Skoog)medium without ammonium nitrate could induct flowering earlier for about3-4weeks. Plantmorphology under different culture conditions changed at the same time. Flowering-inducingin this way shortened the cycle of torenia’s life time as a useful characteristic for being amodel plant. And torenia’s scope of research expanded.The result of suspension culture shows that MS medium with supplement of1mg/L6-benzylamino purine (6-BA) and0.1mg/L2,4-dichlorophenoxyacetic acid (2,4-D)efficiently induced rapid-growing and loose calli. The obtained calli were taken as suspensionculture material. The most suitable conditions for the establishment of cell suspension cultureare liquid MS medium with1mg/L6-BA and0.1mg/L2,4-D, initial inoculum of20g/L,shaking speed of100r/min. The suspended particles which were subcultured3~4times thenwere cultured on half strengh minerals of solid MS medium with1mg/L6-BA and0.1mg/L1-naphthylacetic acid (NAA) for inducing regeneration. The buddings came out in3weeksand later complete plants would be obtained. The suspension culture system has importantapplication value in improving studys which take torenia as a model plant.At the experiment of transforming the Lc gene and PAP1gene into torenia, built plasmidwere transformed into Agrobacterium at first,8mg/l hygromycin was suitable for thegenaration screening culture, transformation followed by Agrobacterium-mediated way wasdone and red plants of overexpressing flavonoid pigments were finally picked out. Thosetransformed materials will be used for the next step which containing expression level test andpigment analysis. Further study of metabolism and breed innovation may also be progressedon the foundation of this paper.