The Study Of Fertility In Torenia Fournieri

Torenia (Torenia fournieri Linden.), an important annual flowering ornamental plant oftropical and subtropical areas, is regarded as a novel model plant for study on flowerdevelopment and fertilization with respect to its short life-cycle and long-term florescence.Thus, torenia could be invaluable for scientific research as a commonly used transgenicmaterial. Since torenia transgenic seedling can not produce filial generation, further analysisis incapable to carry out. Based on the previous study in our lab, we aimed to reveal thecausation for infertility of transgenic torenia by studying the indoor torenia pollinationmechanism and the breeding system of transgenie plants and tregenerated plants in vitroculture. so that to provide foundation for further genetic analysis on transgenetic toreniaplants. The main results were as follows:1. Studies on the potted torenia indoor was conducted to elumiate the mechanism of its poll-ination. The results of observing the floerage of the torenia showed that indoor torenia wasprovided with exterior traits of cross-pollination and that didynamous retroflexion was dis-covered after flowering, which was presumed that the mechanism between long stamenturnover and stigma unfolding might facilitate outcrossing. With the observation of toreniaby mic- roscopical observation, SEM, photography, artificial pollination, physiologicaland bioche- mical analysis Toreniafournieri were systematically studied in this thesis,mainly embracing blossom feature, pollen viability and stigma receptivity, change ofrelatively spatial ubiety between stigma and stamen. The results indicated that the pottedtorenia indoor were pollinated by outcrossing, and explained elementarily the reason whytorenia indoor was infertile unless pollinated artificially.2. Studies were conducted on transgenic Torenia seedlings planted in flowerpots aftersubculture, and the results showed that the 9 transgenic plantlets used were notfructiferous but flowered normally, which should be resulted from the lower pollen vigorranged from 9.8% to 30.6%. Furthermore, the observation of SEM made it more clear thatthe aberrant pollen instead of the stigma was infertile, which belonged to the male sterility.3. Laminae of torenia seedlings developed from sterile seeds were used as explants for regeneration. The observation of florescence and reproduction of the transplanted seedlingsafter times of subculture implied that the pollen vigor reduced about 20% compared to thecontrol after subculture for the first time, and low vigor pollens were obtained similar tothose found in transgenic seedlings. The pollen with 84.6-88.9% vigor of F1 resistantseedlings generated from the hybridization between the transgenic seedlings and the wildtype were observed in our experiment.From the data in our experiments, it is concluded that tissue subculture causedmicrospore sterility in transgenic torenia, and the possible reasons related were discussed inthis paper.