| Wheat varity Yanzhan4110was treated with ultra-high-pressure (120MPa) for8hours.During the wheat plant growth and maturation period, five wheat derived lines induced byultra high-pressure of F4genenration had been selected in the field. Agronomic traits,photosynthesis and chlorophyll fluoreseenee, seleeted wheat were analyzed in the paper, andSSR teehnology was applied for genetie diversity. Results showed as followed:(1) Planting in the field, group analysis of harvested materials showed that obviousdifferences between the wheat derived lines induced by ultra high-pressure and Yanzhan4110in population culm number, tillering number, secondary roots, plant height, number of spike,grains per spike, thousand seed weight and yield. Ultra high-pressure can improve number ofspike, grains per spike and yield of wheat. Mutated strains G2and G3were better than controland had on plant height, grains per spike, number of spike,1000-grain weigh and yield, whichwere more excellent mutated strains.(2) Chlorophyll relative content (SPAD value) of five mutagenic derived lines G2, G3,G5, G9, G11were lower than the control at the seeding stage. From joint to heading stage, itscontent and decline were higher than Yanzhan4110. Soluble protein content of five mutagenicderived lines were significantly lower than control at the seeding-joint stage and significantlyhigher than control at the booting-heading stage. At the seeding-heading stage, there weresignificant different among five mutagenic derived lines in term of soluble protein content.Activities of POD, SOD and CAT of five mutagenic derived lines were below the control atthe seed stage, decreasing along development stages seeding-heading stage and highest at theheading stage. Compared with control, differences in MDA content and activities of POD,SOD and CAT existed as well as among five mutagenic derived lines. It showed obviousdifferences between all of mutagenic derived lines in physiological and biochemicalcharacteristics during heading-mature stage as well as wheat derived lines and control.(3) It showed obvious differences between all of wheat mutated strains in photosynthetic characteristics during heading-mature stage. Chlorophyll relative content (SPAD value) of G3,G9and G11were higher than control in different development stages and its decline was lessthan Yanzhan4110, net photosynthetic rate increased considerably during heading-maturitystage. Compare with control(20.1%), G3and G9increased by29.7%,37.4%, respectively.Net photosynthetic rate increased considerably during heading-mature stage. It showed thatthe stomatal conductance of G3was also higer than control from heading to mature stage. Netphotosynthetic rate of G2and G3increased by19.1%,16.0%respectively, which weresignificant higher than control (only was11.3%) during flowering-filling stage. Theintercellular CO2concentration of G2decreased by0.6%comparing that of control decreasedup4.7%during flowering-mature srage,which displayed a marked difference (p<0.05).Stomatal limitation in G2and G3were highest in heading stage. Water utilization ratio of G2and G3decreased along development stages heading, flowering, filling and mature. Netphotosynthetic rate, chlorophyll content, water utilization ratio and yield were significantlyhigher than control along development stages consist of G2and G3which were moreexcellent mutated strains.(4) SSR teehno]ogy was applied in mutant identifieation and seleetion to improve thebreeding effieiency at the early stage.1680pieces of polymorphic clear bands were amplifiedby10pairs of primers combinations, each SSR primer pairs amplified polymorphic locinumber from3-10per with an average4.54allelic variation. Amplification bands of wheatderived lines induced by ultra high-pressure is109-152, polymorphic bands is27-66. Ascompared with control, polymorphic loci of wheat derived lines induced by ultrahigh-pressure was range of21.28%-49.62%. UPGMA showed the resemble index from0.76to0.96. |
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