| Freeze-drying method was applied to royal jelly to study the concentrate and migratory behavior of Fluvalinate residues during the processing technological process, and the processing factor could be received. Then the changes of residues and its quality (Total Sugar, Total Acid) was researched during the process under different storage temperature of the lyophilized royal jelly. The dietary intake of royal jelly was obtained with different weight and sex using the point evaluation model. The study on the dietary exposure assessment about Fluvalinate in royal jelly was researched referring to the residual concentration of the experiment. The main results are as follows:The extraction solvent and eluent was improved according to SN/T2575—2010The Test Method to Multiresidue of Pyrethroid Pesticides about Import and Export Royal Jelly, using Acetonitrile as the extraction solvent and hexane and acetone (9:1, V/V) as eluent, the linear correlation coefficient of standard curve is0.9995, the standard addition recovery rate was82.68-94.70%, and the relative standard deviation was3.49-5.52%, the minimum detection limit was0.008mg/kg, which could satisfy the analysis of Fluvalinate residues in Royal Jelly.The effects of freeze-drying meterials such as water, malt dextrin, Arabia glue and β-Cyclodextrin to Royal Jelly eutectic point were measured by the resistance method. The results showed that, malt dextrin would increase the eutectic point, while Arabia glue would reduce the eutectic point, the eutectic point would improved by using β-Cyclodextrin at a concentration of5%, and with the increase of the concentration of β-Cyclodextrin, the eutectic point was reduced, pH had no effect with the results.Regarding the Fluvalinate removal rate as the index, respectively using water, Maltodextrin, Arabia glue and of β-Cyclodextrin as the Filtering solvent, the result showed that water of pH8had maximum removal rate, and the Fluvalinate in the filter residue was0.0032mg.Regarding the Fluvalinate removal rate as the index, water of pH8freeze-dring royal jelly sample showed the highest removal rate, which was7.96%, and the Fluvalinate in the filter residue was maximum.12kinds of royal jelly freeze-drying powder residue samples were obtained, the sample filtrated with the concentration of-11%of Maltodextrin which had a minimum concentration of Fluvalinate residues after freeze drying was selected, storged at the temperature of-26℃,4℃,15℃,25℃for0d,15d,30D,45D,60d, the other11samples were stored at the temperature of-26℃,4℃,15℃,25℃for0d,6D,12d,18D,24D,30d, the results showed that,12kinds of freeze-drying powder of Fluvalinate in low temperature conditions remained stable, but the sample filtrated with the concentration of11%of Maltodextrin would reduce during storage at25℃for60d, the acidity increased while the storage temperature incresed, and the total sugar decreased while the temperature increased.Without considering the processing factor, the Fluvalinate chronic risk value of royal jelly%ADI was less than100, belonged to the safe level, while the male chronic risk value%ADI was0.0019-0.0091, and the women chronic risk value%ADI was0.0015-0.0101, and the higher the age, the higher the%ADI. Considering the processing factor, firstly using the worst case of dietary exposure assessment, selected the maximum processing factor with the concentration of0.5%Arabia glue for dietary exposure assessment, the results showed that, the Fluvalinate chronic risk value of royal jelly power%ADI was less than100,, belonged to the safe level. the male chronic risk value%ADI was0.0029-0.0054and the women chronic risk value%ADI was0.0032-0.0054.Considering the processing factor, people chronic dietary risk value (%ADI) were less than the considering processing factor after18years old. |
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