| Three feeding trial were conducted in indoor circulating system to investigate theeffects of fish meal replacement by mixed animal and plant protein on growth andnonspecific immunity of the pacific white shrimp (Litopenaeus vannamei). The resultswere summarized as follows:1. The present study was conducted to evaluate the growth and nonspecificimmunity of Pacific white shrimp in response to the replacement of fish meal by mixedplant protein MPP(canola meal, rice protein concentrate, corn gluten meal with ratio5:3:2). Six isonitrogenous and isolipdic experimental diets were formulated with0,20,40,60,80,100%replacement of fish meal with MPP on a protein basis. Shrimp withinitial weigh0.22±0.01g grew during8-week experimental period. No significantdifference in survival rate was found between the groups,20%replacement of fish mealwith MPP demonstrated the best growth among the diets, with special growth rate SGR6.06±0.04%/d, no significant difference in SGR was observed between full fish mealgroup and80%MPP inclusion(P>0.05). Feed converse rate FCR and protein efficiencyrate PER were slightly better in D20than other diets, FCR was not affected by any ofthe test diets. D100and D40showed the highest superoxide dismutas SOD activity inserum,43.62±2.53U/mg、40.41±1.67U/mg respectively, there is no significantdifference among other diets, the catalase CAT showed the max activity in D20%andD80%, while D60%have the lowest activity, activity of the phenoloxidase PO werehigher in D40%, D60%and D80%, no significant differences were observed betweenthe rest.2. In present study, it evaluated the growth and nonspecific immune characteristicsof the Pacific white shrimp in response to the replacement of fish meal in shrimp dietwith increasing levels of mixed animal protein(Poultry by-product meal, blood meal,further meal, with ratio6.5:2:1.5), shrimp with initial weight0.30±0.01g werestocked into250L indoor experimental system and reared in a8-week period. Fiveisonitrogenous (37.81%±0.14%) and isolipdic (8.81%±0.23%) experimental diets were formulated with0%,30%,50%,70%,90%MAP relacement of fish mealrespectively. There is no significant difference between the groups in survival rate, SGR,FCR and PER(P>0.05), the shrimp fed on the70%MAP inclusion exhibited the best ingrowth, while the D90showed the lowest growth. Carcass composition differed slightlyamong groups. Activity of superoxide dismutase SOD and the catalase CAT showed thedown then up trend with the increasing levels of MAP inclusion, D50showed thehighest activity; lysozyme activity had a up then down trend with MAP replacement,while D50had the lowest activity, contrary to the SOD and CAT.3. The study of nutritional value of Yellow mealworm (Tenebrio molitor) meal(YMM) as an addictive in diet was conducted, Pacific white shrimp (Litopenaeusvannamei) initial weight (0.30±0.01g) were stocked into250L indoor experimentalsystem and reared in a8-week period to evaluate the growth response, carcasscomposition and nonspecific immune characteristics of the shrimp. Four isonitrogenous(37.81%±0.14%) and isolipdic (8.81%±0.23%) experimental diets were formulated with0,1.5%,3.0%,4.5%YMM respectively. The survival rate of shrimp fed the dietscontained3.0%and4.5%YMM were significantly lower than that fed on the controldiet (P<0.05); the control diet group exhibited the best in growth, food converse rateand the protein efficiency rate, significantly higher than that of4.5%inclusion of YMM(P<0.05); no significant difference was observed in carcass composition and the activityof superoxide dismutase SOD(P>0.05); the catalase CAT showed the highest activity inthe4.5%group and the lysozyme activity had a slow up trend with the inlusion of theYMM. |
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