The Impacts Of Herbicide-resistant Transgenic Rice With Bar Gene And Glufosinate On Brown Planthopper, Nilaparvata Lugens (Stal) And Cyrtorhinus Lividipennis(Reuter)

Undesired effects occurring in the transgenic rice nurturing process will change gene products or merabolites or phenotypic traits in herbicide-resistant transgenic rice, and these changes may affect agricultural pests and their natural enemies. In this study, the Nilaparvata lugens (Stal) which seriously affect rice production and their predators Cyrtorhinus lividipennis(Reuter) as the indicator species of non-target organisms, and to investigate the impact of herbicide-resistant transgenic rice Bar68-1and glufosinate on feeding behavior, oviposting behavior, nymphs growth and development, adult fecundity, egg hatchability of BPH; the impact of herbicide-resistant transgenic rice Bar68-1on growth and development, functional response, numerical response, adult longevity and predation of C. Lividipennis; finally, to determine the virulence of glufosinate of BPH and C. Lividipennis. The study will clarify whether the herbicide-resistant transgenic rice Bar68-1and glufosinate have the effect on BPH or C. Lividipennis. The main results are as follows:1. There were no significant differences between Bar68-1and D68on feeding selectivity, honeydew excreted and oviposition preference of BPH, while the viposition selection ratio of D68was lower than that of TN1; There were no significant differences between the treatments of glufosinate and water control on selectivity and honeydew excreted of BPH feeding except oviposition preference of BPH, while it was significantly lower in the number of egg mass, total number of eggs and oviposition selection ratio for the treatment of glufosinate than that of water. 2. Resistant of transgenic rice Bar68-1and parental rice D68to the BPH was evaluated using the Modified Seedbox Screening Technique(MSST). At the seedling stage, Bar68-1had resistance to BPH and expressed short-durable resistance about4days and D68had middle-resistance to BPH and expressed middle-durable resistance about5days.3. There were no significant differences between Bar68-1, D68and TN1on nymphal development, fecundity, egg hatchability of BPH, while the adult longevity of TN1is significantly longer than that of D68but no significant differences comparing with Bar68-1. The population trend index(Ⅰ) was followed by TN1>Bar68-1>D68. There were no significant differences between the treatments of glufosinate and water control on nymphal development, fecundity and egg hatchability of BPH, the population trend index(Ⅰ) was lower than that of the control.4. Rice stem dipping method was used to test the toxicity of glufosinate to BPH and C. Lividipennis, the test showed that the LC50values were4.4876g/L(volume fraction was2.2438%) and0.555g/L(volume fraction was0.2775%) respectively, The test showed that glufosinate with0.5%volume fraction had weak toxicity to BPH, while strong to C. Lividipennis.5. The effects of Bar68-1(transgenic rice), D68(parental rice), TN1on C. Lividipennis affter fed on eggs of BPH were investigated. The result showed that the nymphal duration of Bar68-1was longer than that of D68and TN1, while there were no significant differences between D68and TN1. The orders of female ratio of C. Lividipennis were TN1>Bar68-1>D68, but there were no significant differences among the fecundity. There were differences among the population parameters of varieties of C. Lividipennis. Specifically, the orders of its net reproductive rate, finite rate of increase, intrinsic rate of increase on different varieties were TN1>D68>Bar68-1, the orders of its time of double population were Bar68-1>D68>TN1.6. The functional response and numerical responses of C. Lividipennis(nymph and adult) that was reared on different rice varieties with the eggs of BPH were conducted in laboratory. The functional response were belonged to Holling Type II equation and increased with the increase in the density of eggs of BPH, then tends to be stable. The numerical responses were belonged to Beddington model, and the development was accelerated with the increase in the density of eggs of BPH, then tends to be stable. When the density of egg was1, the nymphy starting from the2nd instar would not be able to growth and development. The functional response of the1st,2nd,3rd,4th,5th instar’s orders were Bar68-1>D68>TN1, Bar68-1>TN1>D68, TN1>Bar68-1>D68, TN1>Bar68-1>D68, Bar68-1>TN1> D68respectively. The orders of functional response of female were TN1>D68>Bar68-1. The fastest development rate was TN1, in addition to the5th instar.