Genetic Analysis For The Progenies From The Cross Saccharum With The Hybrid Of Erianthus Arundinaceus(Retz) Jeswiet And Saccharum SpontaneumL

The filial generations of Erianthus arundinaceus(Retz) Jeswiet and Sacchsrum spontaneumL.have called GXAS07-6-1.When the GXAS07-6-1crossed between different populations is the most common means to enrich genetic variation. In this paper, the main research work as following. Frist, the preliminary analysis for the progenies chromosomes from the cross of Saccharum with GXAS07-6-1. Sencod, the identification of backcross BC1. Third, with the significant difference and correlation analysising for the main characters of BC1generations. At last, used the AFLP molecular markers for Erianthus arundinaceus(Retz) Jeswiet and Saccharum spontaneumL. to analysis the wild genes transfer in them progenies. The results of the study are as follows.1、The results demonstrated that the progenies chromosomes between the Saccharum and GXAS07-6-1probably inherited in the way of n+n. Among them, the somatic chromosome number of the hybrids GXASF108-1from the cross of Yuetang93-159(female parent,2n=102) and GXAS07-6-1(male parent,2n=62) was82(2n=82), and that of the hybrids GXASF108-3from the cross of Guitang02-761(female parent,2n=98) and GXAS07-6-1(male parent,2n=62) was80(2n=80).2、The backcross BC1were analyzed by using SSR markers. The results showed that there32backcross were the true identified by using two pairs of SSR primers.The backcross from the cross of GXASF108-1-1and Co649were GXASBC111-9.The backcross from the cross of GXASF108-3-2and Guitang05-2743were GXASBC111-2.The backcross from the cross of GXASF108-1-11and Co649were GXASBC111-8.The backcross from the cross of GXASF108-3-11and Guitang05-2743were GXASBC111-3.3、The high efficiency and stability of AFLP technical system was set up. The samples DNA was completely digested by3ul enzyme EcoR Ⅰ and Mse Ⅰ.The preliminary amplification optimized reaction conditions was:0.4μL dNTPs(20mmol·mL-1),1.6μLMg2+(25mmol·mL-1),2μLEcoR Ⅰ-P (5pmol·mL-1),2μLMse Ⅰ-P(5pmol·mL-1),1U Taq DNA polymerase (1U·μL-1), DNA template diluted10times,2μL10×PCR Buffer,8μL ddH2O. and the selective amplification optimized reaction conditions was:0.4μLdNTPs(20mmol· mL-1),0.8μLMg2+(25mmol·mL-1),1μLEcoR Ⅰ-a(6pmol·mL-1),1μLMse Ⅰ-b(6pmol·mL-1),3U Taq DNA polymerase (1U·μL-1), DNA template diluted20times,2μL10×PCR Buffer,7.8μLddH2O。4、The result showed that the Erianthus arundinaceus(Retz) Jeswiet and Saccharum spontaneumL.amplification loci was very rich by using the AFLP molecular markers, but the specific site passed to the offspring is low of total site, only6.1%and4.6%. The selected in the F1generation for parents would affected the wild genes transfer to BC1. With the increasing of generation, the trend will be reduced gradually in the offspring, which Saccharum spontaneumL would slower than Erianthus arundinaceus(Retz) Jeswiet.5、In the main yield characters and brix of BC1generations with the significant difference and correlation analysis, the result showed that the three characters of the brix, plant height and stem diameter have closed to the control varietys, and have not significant differences.It can be selected six materials into the next phase of Saccharum hybridization breeding/The varieties of BC111-2-18, BC111-2-16, BC111-3-2, BC111-3-13, BC111-3-16,11-3-27are good behavior in sucrose and yield.