Isolation And Identification Of Bacteria From Snakes

Nowadays special snakes farming has become the emerging industry to get rich in rural areas of Guangxi. But snakes diseases are also increasing with the expansion of the scale of farming and the change of culture way, which seriously affect and restrict the size of the snakes breeding, the production levels and economic benefits. In order to protect the healthy development of Guangxi special snakes and survey the pathogen of bacterial disease of snakes, in the study the heart, liver, lung and other solid organ of the disease snakes were inoculated on blood agar medium under sterile conditions after bacterial isolates, cultured24h at37℃, then30strains were isolated. Including13strains which were not purified and cultured and17strains can be purified and cultured, Gram stain of the latter showed13strains are Gram-negative bacteria and the other4strains are Gram-positive bacteria. Purified17strains exhibit different growth morphology on the nutrient agar medium, SS nutrient agar medium and MacConkey nutrient agar medium.Bacterial genomic DNA were extracted, separated and purified by PCR, universal primers16S-1492R and16S-27F of bacterial16S rRNA sequences were used to amplified and sequence,16S rRNA gene sequence of strain are obtained. Homology search of sequencing sequences were carried in the Genbank database by the NCBI-BLAST software to initially identified the species of the bacteria. Then further determined the bacterial species according to the characteristic reaction of the strains in the biochemical reaction tube.Identification results showed that there are5E. coli, one Morgan coli,3Pang Proteus, one Proteus mirabilis,2Enterococcus faecalis, one arthrobacter, one Pseudomonas aeruginosa, one Stenotrophomonas an oligotrophic Aeromonas, one Salmon ella enterica and one aerogenes among the17strains. Isolation and identification of source of bacteria provide the basis and reference for the determination of its virulence, clinical therapy, disease prevention and treatment.