Molecular Identification And Comparative Analysis Of New Varieties In Resistance To Tomato Yellow Leaf Curl Virus (TYLCV) Discase In Ningxia

Tomato yellow leaf curl virus disease (TYLCD) is a destructive disease of tomato, which spread rapidly and is seriously harmful. The suspected cases of the disease was first found in the autumn of2011in Ningxia Horticultural Industry Park. To ensure the safe production of tomato in Ningxia area, the occurrence of TYLCD and identification of the samples of Ningxia area were investigated, and the resistance of new TYLCD-resistant varieties in the spring and autumn growing season were further compared. The results showed as followings:1. The occurrence of TYLCD in five main tomato cultivation areas in Ningxia was investigated. The results demonstrated that these areas occurred the suspected disease in different degrees, suggesting that the disease have already spread in the Ningxia.2. The samples collected from suspected TYLCD-occurred areas were detected using PCR. Results from PCR identification displayed that6tested samples were infected with TYLCD.3.35tomato varieties (include27new TYLCD-resistant varieties) were compared in the spring growing season, and5big pink tomato varieties, including "Zhuofen225","Zhuofen226","Ouguan" and "Kuke","Afenda", were screened with good performance.4big red tomato varieties including "Zhuokang236","T-5054","Ayisha" and "Qidali" performed better. In addition,1cherry tomato "Qianfen1106" displayed good resistance. Bemisia tabaci is not frequently spreading in the spring and the management measures are in time, so the tested varieties were not infected TYLCV.4. Ten performed better varieties of the spring growing season and the other2varieties (recommended by Ningxia Jiaheyuan Seed Co., Ltd.) in the autumn growing season were compared. The results demonstrated that10varieties performed well in the autumn growing season, and showed high level of resistance to TYLCV. In2varieties recommended by Ningxia Jiaheyuan Seed Co., Ltd,"Fenguo709" displayed high resistance to TYLCD, but growing, yield and quality performance is relatively poor; R8088showed very low resistance to TYLCD. So the two varieties are not suitable for promote and planting in the Ningxia.5.A set of suitable facilities in Ningxia tomato TYLCD disease control measure under greenhouse were summed up based on before the onset of prevention and after the onset of treatment.