Preliminary Studies On Inducing Effect Of Fluoride On Micro-dysbiosis Of Intestinal Microecosystem In Mice

The microecology is an emerging discipline that studies the structure, function, and the relationship with host, with emphasis on the micro-eubiosis, micro-dysbiosis and microecological adjustment of the normal microbial flora. Intestinal microorganisms with a most complex species and structures are of great significance in the research on microecological principles and laws of disease occurrence and development.Much attention was paid to the intestinal microecology in recent years, however, few studies of the effects of fluoride on the human and animal gastrointestinal normal floras. In this experiment, changes in the mouse gut micro-eubiosis induced by fluoride as the main content. The effect of fluoride on the numbers and structures of inherent flora were studied by plate culture counting method after established the animal model of fluorosis by adding sodium fluoride in drinking water. The experiment includes the changes of mainly inherent flora of feces, intestinal membrane flora and colonization resistance (CR). On the basis of the dynamic changes of mice intestinal microbial flora after application of fluoride, the author carries out researches on the difference of susceptibility between the control group that explores the non-fluoride and treated group via a method of infection in mice with Salmonella enterica.The results indicated that the number of intestinal flora and intestinal membrane flora gradually reduced with the increasing in the concentration of fluoride and the extending of the time. The number of bacteria in the feces showed the trend that the first rising after declining with the increasing in fluoride concentration and the extending of time infected Salmonella after treated with fluoride. The number of all the detected bacteria were been rising for Salmonella group. The rates of bacterial translocation were increased caused by Salmonella infection after the fluoride treatment.These findings suggested that fluoride may have some effects on micro-eubiosis of the intestinal flora, and mice may get higher susceptibility of Salmonella enterica by the application of fluoride. From microecology point of view, we can speculate that the animals living in the high fluorine regions are more susceptible to certain infectious diseases, particularly infection caused by the parenteral route.