Screening Of Efficient Lignocellulose Degrading Microbial Community And Its Utilization To Animal Feces

Agricultural waste resources such as animal dung and straw are extremely rich inour country, and it is difficult to achieve effective use in production and had become amajor resource of pollutant in environmental safety. For this reason, acquire microbialagent which can use these waste resources efficiently is a positive thing to theagriculture, animal husbandry sustainable development. A group of lignocellulosedegrading microbial community was screened from soil sample of cattle and chickenfeces compost based on restrictive training technology in this study. The compositionand characterization of this microbial system was identified by using the solid platingculture method, and the degradation capability of lignocellulose microbial communitywas determined by adding different level of filter paper. Without chemical pretreatment,wheat straw, rice straw and corn straw, were used to determine the degradation andresistance microbial contamination capability of lignocellulose microbial community byusing weight loss method, and the ability of this microbial community to utilize anddeodorize livestock and poultry excrement was evaluated when air-dried animalexcrement was utilized as only source of nutrition. The major research results are asfollows:1. The cellulose microbial community obtained in this study could decomposedfilter paper into paste on the48hours culture.2. The decomposition rate of this microbial community decompose filter paper is94.95％under6days continues cultivation,8fungal strains,6bacterial strains and3actinomycetes were isolated and purified from this cellulose microbial community, butno decomposition ability to cellulose when these strains were incubated into each otherartificially.3. It were inoculated with cellulose decomposition bacteria, and consistently addedfilter paper under14days cultivation (continue adding filter paper with0.5%additionwhen it were decomposed into paste), the decomposition weight of filter paper was12.97g, and the degradation rate was83.62%in sterile culture condition (total additive amount of filter paper were15.52g), the decomposition weight of filter paper was13.94g, and the degradation rate was89.54%in impurity bacteria culture condition (totaladditive amount of filter paper were15.57g). In high doses (15g filter paper was addedwith the rate of1.5%at one time in preparation process of culture fluid), however, thedecomposition weight of filter paper was12.09g, and the degradation rate was80.61%in sterile culture condition, and the decomposition weight of filter paper was13.1g andthe degradation rate was87.94%in impurity bacteria culture condition.4. The weight of filter paper, wheat straw, rice straw, corn straw were degraded by47.00%,48.62%and50.21%under6days sterile cultivation, however, when it wereinoculated in impurity bacteria culture under6days cultivation, the weight of filterpaper, wheat straw, rice straw, corn straw were degraded by42.14%,44.99%, and53.74%respectively.5.100mL excrement nutrient solution were prepared with5g pig manure,3gchicken manure as only source of nutrition respectively, and added0.35%filter paper,the cellulose microbial community decompose filter paper into paste at3rd and4.5thdays, the scale for odor intensity can be reduced significantly (weak) compared tocontrol group (strong) at8th days.Lignocellulose microbial community isolated by this study had strong andcontinuous capability to degrade cellulose, which could efficiently decompose strawcellulose material without chemical pretreatment, and quickly degrade cellulose usinganimal excrement. Moreover, it also has strong resistance to undesired microbes anddeodorization capability, could be used in innocuity treatment and resource utilizationof agricultural wastes potentially.