Morphological Differences And Genetic Variation Of Different Geographical Groups In Eleutheronema Rhadinum

Eleutheronema rhadinum, a lively and warm temperate marine fish, generally located in the South China Sea and East China Sea. The advantages are fast growth, meat tender and delicious, and a wide range of salinity adaptation. It can be developed a Releasing objects and proliferation to be suitable for the breeding of the East China Sea area and the Yangtze River estuary, development and application prospects. It has been the FAO as the focus on the promotion of Mari culture fish species, contribute to the Endangered Species reason of the prediction of the fate of species. The genetic diversity of animals and plants are valuable genetic resources. Depth understanding of the genetic diversity is not only the foundation of modern plant and animal genetic breeding in order to meet the human needs change in the future, but also to cultivate appropriate varieties in preparation and it is an important part of the biodiversity. Scientific basis to develop a reasonable protection:the genetic diversity of animals and plants in a sense is a valuable genetic resource. In this paper, using multivariate morph metrics methods and AFLP, Eleutheronema rhadinum of four different geographical groups as the research object, morphological differences and genetic diversity of the of Eleutheronema rhadinum of four geographic groups, screening in addition to the specific genes associated with growth bands, and further understand the genetic characteristics of the Eleutheronema rhadinum population differentiation, has reached the biological basis for the carp seed protection and habitat management.1. Morphological variation of Eleutheronema rhadinum in four geographical populations along china coastTotal144samples of Eleutheronema rhadinum species were collected from the Huang Sea, the East China Sea and the South China Sea. Due to the widespread and ambiguous population structure of Eleutheronema rhadimlm species, three multivariate analysis methods were used to comparably investigate the morphological variation of species. By the cluster analysis, it showed the highest morphological similarity between species of Chongming and species of Qidong, also between species of Zhanjiang and Qionghai. In the principal component analysis of32characters of general and frame morpholog, variation in the area between head and back account for the largest contribution ratio. Subsequent discrimination functions were established by means of stepwise discrimination method, with the general prediction accuracy of84.7%. Own study reveal that variation was emerged in these four geographical species. Based on these characters of variation we can effectively distinguish species using three multivariate analysis methods.2. Screening molecular markers associated Rapid growth using AFLP in Eleutheronema rhadinumAFLP technology can be applied in the genetic breeding of aquatic animals, using molecular marker technology to study the genetic background, filter economic traits closely related molecular markers, can be achieved in the breeding of new varieties of pre-selection of good economic traits, so as to speed up the breeding process, breed new varieties. Screening molecular markers associated rapid growth using AFLP. In this experiment, the farming groups as the research object, selection of fast growth, showed significant growth advantage. To Analysis the genetic difference among the fast-growing group and control group. Using28pairs of primers for the two groups, a total of56individuals were amplified fragment length polymorphism analysis,1015bands were amplified, and each pair of primers34with on average,605polymorphic bands, Polymorphism was59.6%. After analysis of Popgene soft ware found that the rapid growth of population genetic diversity is slightly higher than that in control group, germless resources in good condition. Screened may be associated molecular markers and growth traits, has laid the foundation for the Eleutheronema rhadinum growth of gene mapping, molecular marker-assisted breeding.3. Application AFLP technology analysis the genetic diversity of different geographical populations of Eleutheronema rhadinumEleutheronema rhadinum, a high valued freshwater fish, is wildly spread. To investigate and understand its overall genetic background, AFLP methods were performed to analyze the genetic characteristics and divergences of Eleutheronema rhadinum from Jiangsu, Guangdong, Shanghai, and Hainan. Total246bands were scored in120individuals with128polymorphic bands (53.4%).In our study, group ZJ possess the most amplified loci and the highest polymorphism rate. Nei’s genetic diversity index share the same change trend with Shannon diversity index’ s (CM<QD<QH<ZJ).Genetic variation of4populations were analyzed by AMOVA method. The average fixation index (Fst) was0.1012. Variance among populations was10.12%and90.88%within populations. There was genetic differentiation among populations. The cluster analysis showed that the genetic similarity coefficient among the populations was between0.8918and0.9287, and the genetic distance was between0.0739and0.1145, the genetic distance of group ZJ and group QH was the nearest (0.0739).