The Detection And Monitoring Technology Research For Chloramphenicol And Furazolidone Metabolite

1. Comparison of chemiluminescence enzyme-linked immunoassay with traditionalcolorimetric ELISA for detecting furazolidone metabolite(AOZ) and chloramphenicol(CAP)residues in aquatic products was made in this paper. The research shows: The linear range anddetection limit of CLELA was better than that of ELISA. The recovery rate of two methods haveshown a better close correlation. The detection limit of AOZ was about0.01μg/kg and the linearrange was from0.01to2.56μg/kg using CLEIA. The detection limit of CAP was about0.016μg/kg and the linear range was from0.025μg/kg to6.4μg/kg using CLEIA. The detection limitof AOZ was about0.1μg/kg and the linear range was from0.1to1.62μg/kg using ELISA. Thedetection limit of CAP was about0.05μg/kg and the linear range was from0.05to4.05μg/kgusing ELISA. For CAP in Aspidochirotida japonicus using CLEIA, the precisions of the assaywere satisfactory; RSD was5.5-11.3%and12.3-20.9%, for the intra-and inter-assay precision,respectively. For AOZ in Aspidochirotida japonicus using CLEIA, the precisions of the assaywere satisfactory; RSD was6.6-11.1%and15.6-18.3%, for the intra-and inter-assay precision,respectively.2. In this paper, AOZ and CAP residues of several aquatic of Dalian, Zhuanghe, Yingkouand Jinzhou area were monitoring by CLEIA. The results were as follows:(1)Edible tissues of Anadarauropygimelana, Ruditapes philippinarum, Patinopectenyessoensis, Strongylocentrotus nudus and Apostichopas japonicus for Dalian are no presencefurazolidone metabolite (AOZ) in March, June, September and December,2013. Severalshellfishes for Zhuanghe, Yingkou and Jinzhou are no presence furazolidone metabolite (AOZ)in November,2013.(2)There are different levels chloramphenicol residues for Ruditapes philippinarum,Anadarauropygimelana, Patinopecten yessoensis of Dalian area. In samples collected in March,CAP average content of Ruditapes philippinarum is0.0893μg/kg; CAP average content ofAnadarauropygimelana is0.069μg/kg; CAP average content of Patinopecten yessoensis is0.0741μg/kg. In samples collected in June, CAP average content of Ruditapes philippinarum is0.0907μg/kg; CAP average content of Anadarauropygimelana is0.061μg/kg; CAP averagecontent of Patinopecten yessoensis is0.0657μg/kg. In samples collected in September, CAPaverage content of Ruditapes philippinarum is0.1166g/kg; CAP average content ofAnadarauropygimelana is0.0676μg/kg; CAP average content of Patinopecten yessoensis is0.072μg/kg. In samples collected in December, CAP average content of Ruditapesphilippinarum is0.1444μg/kg; CAP average content of Anadarauropygimelana is0.0693μg/kg;CAP average content of Patinopecten yessoensis is0.076μg/kg. CAP residues in Ruditapes philippinarum’s body tended to increase, content of Anadarauropygimelana, Patinopectenyessoensis changed0.076μg/kg。(3)Chloramphenicol residues was detected in varying degrees in edible shellfish forZhuanghe, Yingkou and Jinzhou areas. CAP average content of Sinonovacula constricta is0.201μg/kg; CAP average content of Patinopecten yessoensis is0.108μg/kg; CAP average content ofMactra veneriformis deshayes in reeve is0.562μg/kg; CAP average content of Saxidomuspurpurata is0.229μg/kg; CAP average content of Crassostrea gigas is0.435μg/kg. CAP averagecontent of Ruditapes philippinarum, Mactra chinensis philippi, Meretrix meretrix, Chlamysfarreri is0.205μg/kg,0.298μg/kg,0.530μg/kg,0.107μg/kg, respectively. CAP average contentof Mactra chinensis philippi, Scapharca broughtonii, Ruditapes philippinarum is0.199μg/kg,0.105μg/kg,0.810μg/kg, respectively. There are Chloramphenicol exceeding phenomena inCrassostrea gigas and Mactra veneriformis deshayes in reeve of Zhuanghe region, Meretrixmeretrix of Yingkou region, Ruditapes philippinarum of Jinzhou region.3. An direct competitive enzyme immunoassays has been developed using a molecularlyimprinted polymer instead of an antibody. The Chloramphenicol Molecularly Imprinted Polymerwere immobilized on96well plates with0.1%methanol and water solution of polyvinylalcohol(PVA). In a direct competitive mode, the concentration of chloramphenicol was measured,using horseradish peroxidase as a marker, with hydrogen peroxide (H2O2)-Lumino for thechemiluminescence system, with Chloramphenicol Molecularly Imprinted Polymer capturechloramphenicol. The use of imprinted polymers instead of antibodies are difficult to obtainexpensive or poor long-term stability has a good application prospect. Calibration curvescorresponding to annalyte concentrations ranging from0.15to4.80μg/kg were obtained. Thelinear equation is y=-3336.96x+18165.06and the correlation coefficient is0.98. The detectionlimit of AOZ was about0.10μg/kg and recovery is85.3%~92.6%. For CAP in Aspidochirotidajaponicus, the precisions of the assay were satisfactory; RSD was under9.2%and16.6%, for theintra-and inter-assay precision, respectively.