The Study Of Lactobacillus Saliva On Health And Intestinal Flora In Broilers

Lactobacillus salivarius is isolated from human intestinal probiotic strains.Lactobacillus salivarius is edible species.on the issuance of the notice of the list ofstrains used in food " in the Notice of the General Office of the Ministry of Healthannounced on April22,2010. However,as most of the microbial cells,Lactobacillussalivarius’activity is easily reduced or even lost under the action of externalstress,and thus lose their effectiveness.Accordingly, the efficient probiotic activityprotection technologies have an important significance to maintain and promote theuse of the efficacy of the preparation of Lactobacillus salivarius. Microencapsulatedtechnology developed in recent years,wich is a great potential for cellimmobilization technology.its advantage lies in probiotics coated by wall materialwith the external environment isolation, under appropriate conditions, wall materialdissolves, the release of probiotics play a role.Microencapsulated probiotics couldnot only prevent the inactivation of bacteria to improve the stability of probioticsproducts, processing and gastrointestinal environment, but also to prevent its activedestruction, so that more bacteria could reach the intestinal tract, t and really piay theroleof health care and treatment.The purpose of this study is to research the effects of microencapsulatedLactobacillus salivarius (Lactobacillus salivarius subsp. Salivarius) on growthperformance, serum antioxidative capacity and IgG level in broilers. A total of2521-day-old AA broilers were randomly assigned to six dietary treatments(Ⅰ-Ⅵ）with6replicates per group and7broilers per replicates. The treatments including a basaldiet (Ⅰc ongtol group），antibiotic group（Ⅱ,50mg/kg），a low dose of Lactobacillussalivarius group（Ⅲ,108CFU/kg basal diet）, a high dose of Lactobacillus salivariusgroup（Ⅳ,109CFU/kg basal diet）, a low dose of microcapsulated Lactobacillussalivarius group（Ⅴ,108CFU/kg basal diet）and a high dose of microcapsulated Lactobacillus salivarius group（Ⅵ109CFU/kg basal diet）.The growth performance,antioxidative capacity and IgG level in broilers were determined when broilers were21-days-old （earlier stage）and42-days-old (later stage). The results showed thatthe daily gain and the feed conversion ratio of broilers got marked improvement,while the Mortality and diarrhea rate got significant decrease during the whole stagein the low dose of microcapsulated Lactobacillus salivarius group when comparedwith that of control group and the feed conversion ratio showed10%（P＜0.05） and6.4%（P＜0.05）decease than the control and the Antibiotics group respectively.Moreover, The low dose of microcapsulated Lactobacillus salivarius group couldsignificantly improve antioxidative capacity of broilers when compared with that ofantibiotics group with results of15.1%（P＜0.05）and13.2%（P＜0.05）increase ofGSH-PX in the serum of21th and42th day broilers and MDA in the serum wasreduced by36.6%（P＜0.05）in the42th day. Finally, The IgG in the serum of the lowdose of microcapsulated Lactobacillus salivarius showed an significantimprovement during the whole stage（P＜0.05）. The dailydiet adding lactobacillussaliva could maintain the balance of bacteria within the chicken caecum.Theseresults indicate that the low doses of microcapsulated Lactobacillus salivarius (108cfu/kg diet) could improve the production performance of broilers, and improveserum antioxidant ability and immune function, improve intestinal health, at thesame time, maintain the balance of intestinal flora.so the Lactobacillus salivariusafter microencapsulation can be used as the potentially substitutes of antibioticduring broiler feed.