Study On Secondary Metablites From Two Marine-Derived Fungi Penicillium Oxalicum And Aspergullus Nidulans

Thirty-one compounds (including one new compound and two isolated from the genus ofAspergullus nidulans for the first time) were isolated and purified from two marine-derivedPenicillium oxalicum and Aspergullus nidulans by using various chromatographic methods(silica gel column chromatography, ODS reverse phase gel column chromatography, gelchromatography and preparative HPLC). Their structures were elucidated by extensivespectroscopic analysis (1D and2D-NMR、MS) and Computational chemistry methods (VCD,ECD, OR).Twenty-four compounds were obtained from the fermentation broth of the strainPenicillium oxalicum including one phthalide derivative with a new stero configurationnamed (3R,4S)-3,4,6,8-tetrahydroxy-3,4-dihydronaphthalen-1(2H)-one (PX-1). The absoluteconfiguration of PX-1was determined by using three spectroscopy methods (VCD、ECD andOR). In adition, other monomer compounds include eight steroids, three coumarins, fourbenzene derivatives, two cyclic dipeptides, two nucleotide bases and two isoflavones. Thestructures of these monomer compounds were identificated through NMR, HRMS andprevious reported literatures.Seven compounds were obtained from the fermentation broth of the strain Aspergullusnidulans, a fungus isolated from a deep sea water sample collected in the western Pacific,including a sugar alcohol ester (GC-2), hexane-1,2,6-triol (GC-6) and Chrysogeside B (GC-7)that in this study were the first time isolated from the fungus genus Aspergullus. Thestructures of these compounds were established on the basis of spectroscopic analysis and therelative reported literatures. The types of these compounds were various including nucleotidebases (GC-1,GC-3), cyclic peptide (GC-4), succinic acid (GC-5) and Monosaccharide brainglycoside (GC-7).