The Distribution And Filter Participate In Disease Resistance Of WRKY Transcription Factors In Maize

WRKY genes as an important class of transcription factors in the model plant Arabidopsis and rice, the reported broad participation in a series of physiological and biochemical processes, but the most important function of WRKY genes is involved in the response of plants to biotic and abiotic stress. Currently, Arabidopsis、rice、 maize and some representative plant genome sequencing have been completed, release large amounts of genomic data. WRKY gene family on maize bioinformatics and comparative genome analysis and forecast corn WRKY genes function. Corn is the world’s third largest food crop, after rice acreage and production, yield per unit area ranks first in the world’s crop. In the production of corn diseases seriously affect corn production is an important factor restricting the healthy development of maize production, due to diseases caused by the loss of up to6%to10%, in severe cases more than30%.The experimental maize WRKY gene sequence information system, alternative splicing, zinc finger structure model, chromosome distribution, intron type, collinear gene, functional distance, tissue expression and its bioinformatics and molecular biology combination of screening corn resistant WRKY genes, analytical WRKY gene family in the maize genome-wide distribution and evolution of the law, get some WRKY genes may be involved in the disease control, provide for further high-yield breeding genetic resources. This issue can also be carried out at the same time provide new ideas for the screening of genetic resources. The main results are as follows:1. In search of the genome database, we get125corn WRKY genes. The results show that the sequence alignment, wherein117containing complete WRKY structures domain, six genes containing only WRKY area, two genes containing only zinc finger domain. Complete WRKY genes,16WRKY proteins contain two WRKY domain, the30WRKY protein containing Cys2HisCys zinc finger.2. The117WRKY genes containing complete the WRKY domain, domain phylogenetic tree was constructed. The phylogenetic tree was eight distinct enclave. NTWDs and CTWDs gathered in two different regions; class Ⅱ gathered in five enclave;30class III genes are clustered in the same region.3. Alternative splicing exist in the corn WRKY genes the WRKY domains not necessarily intact alternatively spliced variants. The WRKY domains exist two kinds of introns inserted into the form:A exists in the R amino acid coding region, in the zinc finger the C2H2domain structure in front of the first carbon fifth amino acids, this intron inserted defined as R-type intron; another intron V residue in the zinc finger the C2H2domain structure after the second carbon sixth amino acid, this time0into the intron is defined Ⅴ-type intron.4. In WRKYGQ these six amino acid residues, mutation in R and Q amino acid was observed. However, in most cases, the mutations are changed from Q to E or Q becomes K. Zinc finger structure of the general formula can be expressed as C-Xn-C-XM-HXH, or the C-Xn-C-Xm-HXC. Although the class I WRKY gene NTWDs of and CTWDs n values are conservative, but not the same as the value of m, m value in NTWDs22(addition to ZmWRKY8392) in CTWDs, m=23; class Ⅱ zinc finger more conservative, except in ZmWRKY33(m=22) for6and96(m=24), n is4or5, m is23; in III, n value from5to7, m value from23to34are present.5. We found the125the WRKY class gene distribution on maize chromosome10. Function from the results of the analysis, clustering II c bF value is almost zero. Copy genes show that the rate of evolution of each location with the original to maintain, these duplicated genes with the original gene more inclined to consistency, the WRKY genes evolution, these clusters may inherit more primitive features.6.47WRKY genes in maize roots, stems, leaves, filaments, pollen and seeds in the tissue-specific expression analysis showed that the tissue-specific expression of maize WRKY genes. WRKY gene expression in different tissues differentiated for us to further resolve the corn WRKY gene function.7. The8by rice and maize genome collinearity, combined with the phylogenetic tree screening to6may be involved in maize resistance to disease WRKY genes ZmWRKY12ZmWRKY15, ZmWRKY34ZmWRKY48ZmWRKY83and ZmWRKY87. Further validation of molecular biology, ZmWRKY12ZmWRKY48subject SA induced expression ZmWRKY15the inducible expression by MeJA. 8. In this study, four common fungal pathogen inoculation corn microarray data for further analysis found that maize WRKY genes are widely involved in regulation of the fungal pathogen of the disease,27WRKY genes at least by a fungal pathogen-induce dupregulated,9WRKY at least by a fungal induced down-regulated,5WRKY genes by different fungal infections may be upregulated and down-regulated. For the preceding31WRKY genes clustering analysis showed that they are distributed in Ⅰ, Ⅱa, Ⅱc, Ⅱd, Ⅱe, and Ⅲ. Which WRKYGE WRKYGK WRKYGQ type can participate in the regulation of maize disease. It is noteworthy that after the induction of these four fungi, all corn material able upregulated two genes, located the Ⅱc class of ZmWRKY36and in class Ⅰ of ZmWRKY43. Therefore, we speculated that the two WRKY gene may be involved in maize variety of biological stress regulation process, they may be in the corn resistant regulation has a broad spectrum.