Bioinformatics Analysis Of WRKY Transcription Factor Family And Identification Of Some Genes Related To Stress Resistance In Tomato

Tomatoes have the characteristics of rich nutrition,wide adaptability,easy cultivation and high output,versatility and so on.Therefore,tomato is an important vegetable in the world.and plays a key role in production.However,in the process of tomato growth,it is usually affected by the harsh natural environment,which leads to the decline of yield,so it is very important to study the effect of stress on tomato growth and development.1.Through the bioinformatics analysis,we identified that the tomato Sl WRKY family contains 81 members,divided into 3 groups according to their WRKY domain and zinc finger structure,and the group 2 can be divided into 5 subgroups according to their amino acid sequence.In addition to tomato chromosome 11,there was no WRKY transcription factor,which was distributed on other chromosomes.Only Sl WRKY19,Sl WRKY20 two family members do not contain introns,other members contain 2~5 introns.Tomato WRKY domain is highly conserved,only 10 WRKYGQK mutated,accounting for 10% of WRKY family.2.Tissue specific expression pattern of 6 typical WRKY transcription factor were analysised.We revealed that these genes,in root,stem,leaf,flower and fruit were expressed,but there are differences in expression of different genes in the same tissues,there are differences in expression of the same gene in different tissues of quantity.The expressions of Sl WRKY13 and Sl WRKY24 was higher in roots and flowers,almost no expression in stem;the expression of Sl WRKY31 in root,stem and leaves was higher;the expression of Sl WRKY50 in root,leaves and flower was higher,almost no expression in stems;the expression of Sl WRKY62 in root,leaves and fruit was higher,almost no expression in stems;the expression of Sl WRKY63 in root was higher,almost no expression in stems and fruit.3.The expression of Sl WRKY13,Sl WRKY31,Sl WRKY50,Sl WRKY62 and Sl WRKY63 were u p-regulated first and then decreased gradually in the salt stress treatment,but the expressionof Sl WRKY24 gene was significantly down regulated;in drought stress treatment,Sl WRKY13,Sl WRKY31,Sl WRKY62 and Sl WRKY63 gene expression was up-regulated,and the expressio n of Sl WRKY24 and Sl WRKY50 was down regulated;At low temperature stress,Sl WRKY13,S l WRKY31,Sl WRKY50,Sl WRKY62 and Sl WRKY63 gene expression was up-regulated,and the ex pression of Sl WRKY24 gene was down regulated.4.We studied the Sl WRKY50 which expression was significantly changed in response to abiotic stress by virus-induced gene silencing(VIGR).It was found that the MDA index of the plants under the low temperature stress was significantly higher than that of the control plants,while the Pro and SOD indexes decreased significantly.The results showed that the down regulated expression of this gene could reduce the cold resistance of plants.