| In order to reduce the starch content of flue-cured tobacco in our country,improve related sugars of the chemical composition of the coordination andexploration of starch, amylose and amylopectin content in2010~2012, henanagricultural university in science and education industrial park based on potexperiment method, studied the different light environment carbohydrates during theprocess of tobacco leaf maturity and part of the enzyme activity and the impact ofbotany traits; The field production of tobacco, curing and half drying and curingmethod, upper leaves starch degradation was studied and related enzyme activity’sinfluence on the quality; With double wavelength spectrophotometry determination ofresearch the amylose and amylopectin in tobacco. The main results were as follows:1. Under the different light quality condition, K326varieties of flue-cured tobaccoleaf tobacco in neutral shade colorless membrane compared with red coating tobaccoinvertase activity decreased, activities of amylase, the starch and reducing sugarcontent increased; Blue film processing tobacco leaf lower invertase and amylaseactivity, starch and total sugar content is higher; Green coating tobacco invertaseactivity, and amylase activity is abate, starch, total sugar and reducing sugar contentreduced; Botany traits better show the red film processing, green film, blue film andcompared in dealing with difference. The results of the study to reveal the influence ofthe light environment of tobacco leaf quality laid a solid foundation.2. In different dry yellow time (48,60,72h) ZhongYan under the condition of100and over87of upper leaves compared with control (conventional baking), the threeair processing of tobacco at the early stage of the baking amylase activity increased by30.65%,31.21%and31.21%, respectively. Drying system in the baking process oftobacco amylase activity in advance12h at first, and at the same time achieve the second peak in72h, extend the effective duration of activity of amylase; Compared tothree kinds of drying system processing baking early starch phosphorylase activityreduced by40.01%,42.53%and42.53%, respectively, in the baking process,respectively, have reached first peak in36h and72h second peak. The activity ofamylase and starch phosphorylase has changed, and occurs mainly in yellowing stageand color earlier. Comprehensive comparison of different drying conditions, dryyellow roasted tobacco leaf after60h of amylase and starch phosphorylase activity,starch degradation amount is higher, after baking tobacco leaf starch content is low,water soluble total sugar, reducing sugar content is higher, all kinds of chemicalcomposition more coordination, better appearance quality, higher proportion ofsuperior tobacco leaves, mottled leaves ratio is low, the usability of upper leaves ishigher.3. According to the principle of double wavelength than the color, the choice ofamylose determination wavelength of512nm and512nm, the determination ofbranched chain starch wavelength of560nm and560nm, according to the regressionequation to calculate the content of amylose and amylopectin. Tobacco samples wasevaluated by dual wavelength method of starch content, one can get at the same timeamylose, amylopectin and total starch content three kinds of data, and the provisionsof the determination of the experimental results show that linear and branched chainsample recovery rate is higher. Dual wavelength method using only an absorption cell,and use test solution itself as reference fluid, can eliminate the absorption pool and theerror due to factors such as reference fluid, improves the accuracy of measurement.Method established in this paper through the determination of different types anddifferent quality of amylose and amylopectin content in tobacco leaf and its ratio, andhelp to know the structure of amylose and amylopectin and starch physicochemicalproperties and starch degradation in tobacco baking process and quality of tobaccoleaf form. |
PDF Full Text Request