Study On The Toxicity Of Glyphosate To Stichopus Japonicus

Since it has the characteristic of efficient, broad spectrum herbicide, low toxicity, and easyto decompose, low-residue, low impact on the environment and so on，glyphosate（G PS）has been the world’s most produced and sold pesticide. However, while it is widelyused in the agriculture and bringing enormous benefit to people, a large number ofglyphosate along with rivers, groundwater convergence to the aquaculture waters，glyphosate accumulates in water, it suppresses the production of Fisheries animal, and evenmore injurious to human health. At present, the research home and abroad on the toxicity ofglyphosate on sea cucumber into parameters have not been reported, The purpose of thisexperiment is to research the toxic effects of glyphosate on Apostichopus japonicus, so as toprovide the basis for reasonably used of glyphosate, protecting the production of fishery andimproving the safety of biological.This study based on physiological, biochemical and histological methods, divides intothree parts: acute toxicity test, physiological toxicity test, the histopathology research. Wepreliminary discussed the toxicity effects of glyphosate on Apostichopus japonicus. The testresults as follows:1. Through acute toxicity test, the glyphosate on Apostichopus japonicus48h medianlethal concentration (median lethal concentration, LC50)132.99mg/L,96h median lethalconcentration (median lethal concentration, LC50)97.92mg/L, safe concentration (safeconcentration, Sc)9.79mg/L. According to our grading standards for pesticides, glyphosateis a low-toxicity pesticide.2. Using the method polyarylamidegel electrophoresis (SDS-PAGE), Prepared44.0mg/L、102.7mg/L、161.34mg/L、220.01mg/L concentrations of glyphosateaccording its48h LC50, with the activity of malate dehydrogenase (MDH), esterase (EST),Superoxide dismutase(SOD) of the Apostichopus japonicus muscle and Intestinal asindicator to explore the effect of glyphosate on the isoenzyme expression of Apostichopusjaponicus. Experimental data shows: With increasing of the exposure concentration, theintestinal MDH activity increased at first, then fall; The muscle EST activity increased atfirst, then fall, while it activity of intestinal had been declining with the increasing ofexposure concentration; SOD activity of muscle and intestinal both increased with rising ofdrug concentration. This shows that glyphosate had toxicity on MDH、EST and inApostichopus japonicus expression.3. At this stage, Using histopathological methods we observed the Muscle and intestinalbefore and after Apostichopus japonicus exposed in safe concentration. The results show that: After Apostichopus japonicus exposed in safe concentration, its muscular endodermiscell quantity loose, connective tissue collagen fibers had been destroyed. Intestinal maskepithelial cells shedding, dissolved and disappear. Intestinalfolds round shield defect and even disconnect.