Research On Pathways For Clone’s Establishment Of Plantago Depressa

Plantago depressa is Annual herb plant belongs to Plantago Cass. People like itbecause of its very important edible and medicinal value. The speed of artificial cultivation isslow and wild resources can not meet the needs of people, so it’s particularly important toseek the method for rapid propagation. The purpose of this study lies in the use of tissueculture technology to explore suitable way for Plantago depressa rapid propagation, then theclone of Plantago depressa was established, favorable to the front of a large number ofbreeding, thus protecting the wild resources, and achieve good economic value and ecologicalsignificance. Induce callus differentiation and rootstocks differentiation can both obtainregeneration seedlings of Plantago depressa, the high efficient regeneration system ofPlantago depressa was successfully established, specific results are shown as follows:1.The influence of different conditions on the callus inductionStipes of Plantago depressa were used as explants to induce callus. The results indicatedthat MS+6-BA0.2mg·L^-1+2,4-D2.0mg·L^-1+sucrose30g·L^-1was the perfect medium forcallus induction.2.Different concentrations of6-BA,2,4-D and NAA effect on callus proliferationThe results indicated that MS+6-BA0.2mg·L^-1+2,4-D2.0mg·L^-1+sucrose30g·L^-1wasthe perfect medium for callus inducing; multiplication coefficient were respectively13.7.3.Different concentrations of6BA, KT, ZT and NAA effects on callus differentiationIntegrated all ideal factors, the optimum condition for callus differentiation was1/2MS+6-BA0.2mg·L^-1+sucrose15g·L^-1with constant temperature illumination, the rate ofdifferentiation was100%, each callus differentiated3⁷adventitious buds on average.4.Effects of different materials and conditions on direct differentiationRootstocks, leaves, and stipes without buds of Plantago depressa were used as explantsto induce direct differentiation.The results indicated that rootstocks without buds were thebest one of the three stuffs for direct differentiation inducing without callus, MS+BA1.5mg·L^-1+sucrose30g·L^-1was the perfect medium for direct differentiation induction, growthbuds induction rate is as high as86%.After successive transfer culture28d get a lot of thegrowth of buds, the growth of the average vaccination bud proliferation4.62times.Experimental results showed that AgNO3is not suitable for Plantago depressa’s explanttissue culture, GA is beneficial to petiole elongation, no promoting effect on differentiation.5.Effects of different conditions on rooting of differentiated budsThe results indicated that the best medium for rooting of differentiated buds was1/4MS+IAA0.2mg·L^-1+sucrose20g·L^-1,rooting rate was100%, average number of roots from one tube seedling was7.22and average length of root was3.12cm. Taproot stout, rootfor the green and white tender roots grow.6.Refine seedling, transplanting and colonizationClays from garden was the best substrate for transplanting of regeneration plants, thetransplanting survival rate was100%and stable planting survival rate was100%.The tubeseedlings survivaled retained all biological characteristics of the escape Plantago depressawith exuberant growth.