Expression Of Adiponectin On Adipose Tissue And Effects Of Adiponectin On Intramuscular Fat Of Wannanhua Pigs

Wannanhua pigs were superordinary local ones of Anhui province. The geneticqualities and production traits of Wannanhua pigs were special in Chinese local pigs.Adipose tissue were not only the tissue for fat storage, but also the tissue which played animportant role in endocrine, metabolism, controlling body’s energy balance, ets. Up to now,adiponectin was a sole adipokines whose blood concentration decreased with the quantityof adipose organization (tissue) increasing. In this study, Adp and AdipoR mRNAexpression in subcutaneous (SC) and perirenal (PR) adipose tissue was investigated. Andthe inner relationship of Adp and meat quality in Wannanhua pigs was approached. Theeffects of recombinant Adp (rAdp) on intramuscular adipocytes of pigs were also studied inthis paper in order to explore the influence of Adp on meat quality in Wannanhua Pig, andits possible mechanism.1Adiponectin (Adp) and AdipoR mRNA expression in adipose tissues of Wannanhuapigs.Pigs were followed an access to spontaneous feed and water. Pigs were slaughted at90days of age after12hours fasting. The abundances of Adp, adiponectin receptorsR1(AdipoR1), adiponectin receptor R2（AdipoR2） mRNA in SC and PR adipose tissuewere detected by real-time fluorescence quantitative RT-PCR. The results were as follows:(1) Adp mRNA expression in SC adipose tissue was significantly higher than that inPR adipose tissue (P<0.05), while AdipoR2and AdipoR1mRNA expression in SC adiposetissue were significantly higher than those in PR adipose tissue (P<0.01).(2) Significant positive correlation result was between Adp and AdipoR1, AdipoR2inSC adipose tissue(r=0.60, r=0.57, P<0.05）and PR adipose tissue (r=0.31, r=0.37,P<0.05）The results suggest that Adp and AdipoR are expressed in adipose tissue, SC adiposetissue maybe the main target tissue of the Adp.2Effect of adiponectin on intramuscular adipocytes of Wannanhua pigs and itspossible mechanismPrimary preadipocytes were separated from musculus longissimus tissue ofWannanhua pig at5days of age. Cells were cultured to80%confluence. Adipocytes weretreated with0,2and10μg/mL recombinate adiponectin (rAdp) respectively for12h or48h. Oil-red O and MTT methods were used to identify adipocytes and to detect viability of cells. Adp, AdipoR1, AdipoR2, PPARα and AMPK mRNA levels were determined byqRT-PCR. The results showed that the viability of cells had a reduced tendency, anddecreased significantly by2μg/ml rAdp treatment for24h (P<0.05). After rAdp treatmentfor12h, AdipoR1and AdipoR2mRNA expression of adipocytes were significantlyincreased (P<0.05), AMPK mRNA expression of adipocytes was significantly decreased(P<0.05), but all with no dose-dependent. After rAdp treatment for48h, Adp mRNAexpression of adipocytes was significantly decreased (P<0.05), PPARα mRNA expressionwas significantly decreased (P<0.05), but with no dose dependent; while AMPK mRNAexpression of adipocytes were significantly increased by10μg/ml rAdp treatment (P <0.05).In addition, the effects of rAdp treatment on AMPK mRNA expression were blocked byCoumpound C. The results indicated that recombinant adiponectin may depress theviability of intramuscular adipocytes. The effects of rAdp on cultured intramuscularadipocytes may mediated by up-regulating the mRNA expression of AdipoR1, AdipoR2and affecting mRNAexpression of the mRNAexpression of PPAR andAMPK.