Charcateirzation And QTL Analysis For Low-phosphorus Tolerance Related Traits Of Trititrigia Germplasm Shannong303

In this paper, the characters while phosphorus insufficiency of Trititrigia GermplasmShannong303（SN303） and its common wheat parent Yannong15（YN15） were studied, usinghydroponics, vermiculite culture and soil culture method. A F2population derived fromSN303and YN15was developed and used for QTLs analysis of low-P tolerance characters.The main results are as following:1. Under hydroponics conditions, shoot dry matter of SN303is higher than YN15, as4.3%at P10levels and17.9%at P50level, respectively. SN303also had higher phosphorusaccumulation than YN15in low-P level, while it had short root dry matter. While in the low-Plevel, the solution of SN303had lower pH value. HPLC analysis showed its roots releasemore oxalic acid than YN15and XY54.2. While vermiculite culture using different P source, SN303uptakes more P than YN15,especially for KH₂PO₄and AlPO₄conditions. SN303can produce the largest root, and keepmaximum root-shoot ratio while use AlPO₄as the P resourses. For FePO₄and Ca₃（PO₄）₂conditions, these two wheat genotype have no signification difference.3. Under soil culture condition, SN303produced higher shoot dry weight and root dryweight than YN15both in P application and non-P treating; While in non-P treating, SN303is23.9%and71.6%more than YN15for SDM and RDM, respectively. While in P applicationtreating, SN303is19.2%and43.3%more than YN15for SDM and RDM. And the PUE ofSN303is higher than YN15. Soil culture condition, SN303accumulates more P in root andshoot, produces more biomass, compared with YN15.4. With molecular maker analysis, SN303is a translocation line derived from theprogenies of common wheat YN15and Thinopyrum intermedium. The makers barc070-4AL、xgwm513-4B、cau12-7B and barc125-2B amplified corresponding fragment on Thinopyrumintermedium.5. The QTL on low-P tolerance traits were mainly distributed on2A、2B、3B、4A、4B and7B, referring to11QTL clusters, showing pleiotropic effects and including88majorQTLs. The QTL clusters of low-P tolerance related traits were detected around the molecularmarkers barc070and xgwm513connected with to Thinopyrum intermedium.