Molecular And Cytogenetics Identification Of Wheat-Thinopyrum Intermedium Derivatives

Thinopyrum intermedium (2n=42) is a perennial cross-pollinated plant of Elytrigia, itshows immunity or highly resistance on wheat powdery mildew, rusts, yellow dwarf disease,root rot, gibberellic disease and so on. It immune to all Jordanons of stripe rust racescurrently. It provides useful genes for wheat breeding, is earliest to hybridize success withwheat in Elytrigia，is one of the main species in wheat distant hybridization. The studyidentifies6different chromosome combinations which are filial generation of wheat-YuanZhong2derived offspring by cytology, morphology and molecular markers method, aim tolay the foundation for further research and application of these materials.The results obtained are as follows：Combination NZ2W1morphological aspects: loose type plant is10, an tiller average of15, with an average height of111cm, average main spike spikelet is20, the average earlength is9.6cm, all have top Mans, blue-green leaves. morphological characters is Stable.Somatic chromosome number of2n=43. Chromosome configuratiuon at PMC MI are2n=21II+I.Itdoes not resistant to stripe rust. Specific bands Xwmc489and Xwmc331of Thinopyrumintermedium could be amplified in NZ2W1by SSR analysis. Fluorescence signal is notdetected by in situ hybridization.Combination NZ2W2morphological features: loose type plant is10, an tiller average of19, with an average height of118cm, average main spike spikelet is19, the average earlength is11.1cm,they are awnless variety, gray-green leaves. Somatic chromosome numberof2n=43. Chromosome configuratiuon at PMC MI are2n=21II+I,.It does not resistant to striperust.Specific bands of Thinopyrum intermedium Xcfd13could be amplified in NZ2W2bySSR analysis. Fluorescence signal is not detected by in situ hybridization.Combination NZ2W3morphological characteristics: loose type plant is10, an tilleraverage of20, with an average height of131cm, average main spike spikelet is20, theaverage ear length is11.6cm, all have top Mans, blue-green leaves. Somatic chromosomenumber of2n=42, Chromosome configuratiuon at PMC MI are2n=21II, it inherits good genesThinopyrum intermedium in stripe rust resistance aspect, It shows stripe rust resistance inadult stage survey. no marks could be amplified specific bands of Thinopyrum intermedium inNZ2W3by SSR analysis. Fluorescence signal is not detected by in situ hybridization. Combination NZ2W5morphological features: compact type plant is50, an tiller averageof15, the average ear length is115cm, average main spike spikelet is19, the average earlength is11.4cm, all have short awn, Blue-gray leaves. Somatic chromosome number of2n=42,2n=44,2n=46, Chromosome configuratiuon at PMC M I are2n=21II、2n=22II、2n=23II,28%of plant shows stripe rust resistance in50.9markers could be amplified specific bandsof Thinopyrum intermedium in NZ2W5by SSR analysis. GISH shows that2completechromosomes of2n=44material of combinations NZ2W5somatic cell indicateshybridization signal, it indicates these materials attaches2chromosomes of Thinopyrumintermedium, and forms alien addition lines;4complete chromosomes of2n=46material ofsomatic cell indicates hybridization signal, it shows these materials attached4chromosomesof Thinopyrum intermedium, and forms alien addition lines.Combination NZ2W6morphological features: loose type plant is10, an tiller average of14, the average ear length is120cm, average main spike spikelet is21, the average ear lengthis13.9cm, all have top Mans, Blue-gray leaves. Somatic chromosome number2n=42and2n=44etc, Chromosome configuratiuon at PMC MI are2n=21II、2n=22II etc.6%of plantshowed stripe rust resistance.8markers could be amplified specific bands of Thinopyrumintermedium in NZ2W1by SSR analysis. Fluorescence signal is not detected by in situhybridization.Combination NZ2W7morphological features: compact type plant is10, an tiller averageof14, the average ear length is105cm, average main spike spikelet is20, the average earlength is12.3cm, all have top Mans, Blue-gray leaves. Somatic chromosome number2n=42、2n=46etc, Chromosome configuratiuon at PMC MI are2n=21II、2n=23II etc.23%of plantshows stripe rust resistance. specific bands of Thinopyrum intermedium Xbarc195andXcfd3440could be amplified in NZ2W7by SSR analysis. Fluorescence signal is not detectedby in situ hybridization.