The Effects Of Phytase Transgenic Maize On Rhizosphere Microorganisms And Rhizosphere Soil Active Constituents

As national major projects for transgenic organisms carrying out, more and more genetically modified crops run into the stages of environmental release or preproduction field trial. As a new transgenic maize with proprietary intellectual property rights in our country, trans phytase corn has some advantages such as:improve the utilization rate of feed nutrition, reduce the phosphorus pollution and reduce the production cost et al. Until now, the environmental safety study is rare both at home and abroad. Using parental line Liyu35and conventional line Zhengdan958as control, the effects of phytase transgenic maize on soil fertility, protein content of rhizosphere soil, microbial diversity of rhizosphere soil and horizontal gene transfer were studied. The results were as follows:1. The effects of phytase transgenic maize on function diversity of soil microbial communities were studied using Biolog method. The results showed that richness and diversity of soil microbial population were increased gradually in the corn growth Compared with other two non-transgenic maize, the phytase transgenic maize showed a more abundant community diversity in rhizosphere soil microorganism. Statistical analysis showed that there was no significant difference between phytase transgenic maize and other two non-transgenic maize in Shannon’s index of rhizosphere soil microbial functional diversity index and evenness index, except in Simpson dominance index.2.The result of N、P and K content in root soil showed that though the nitrogen content in soil increased during the straw returned period, but it didn’t make a significant difference in N P and K content during the child bearing period and the straw returned period.3. BCA and ELISA were used for three kinds of maize in baby-bearing period to find out whether the root soil total protein content and phytase enzyme have changed. The result showed that there is a gradually increasing trend for rhizosphere soil total protein of three kinds maizes. Compared with the other two non-transgene maizes, there is no significant difference in the content of rhizosphere soil total protein for the phytase maize though the phytase maize has a higher contentment. 4. Use of Acinetobacter baylyi sp. ADP1construct phytase gene transfer parallel detection model. In order to clarify whether the exogenous gene will transfer into the soil microorganism, the establishment of Exogenous horizontal gene transfer detection system is taking Acinetobacter Acinetobacter baylyi sp and The ADP1which have the strongest ability to accept foreign DNA in the state of nature as indicator bacteria. Treatment for Phytase maize genomic fragments for sonication treatment, it did’t find transformant containing the phytase gene in the the PCR detection of more than106transformants. It is reasoned that Phytase maize or exogenous gene will not or may be transferred with a frequency lower than10-6and moved into microorganisms soil.