Detection Of Conditional And Unconditional QTL For Grain Weight Per Panicle,1000-grain Weight And Related Traits In Wheat

In this study, we use three populations to detect the QTL. The first one is adouble haploid (DH) population comprising168lines deriving from a cross betweenHuapei3and Yumai57; the second is a recombinant inbred line population(RIL1)comprising182lines generated by out crossing the strain Gaocheng9411toShannong01-35; and the last one is also a recombinant inbred line population(RIL2)deriving from Nuomai1and Gaocheng8901which contains256lines. Combinedwith three molecular genetic linkage maps, we have detcetede the QTL for headingdate and floering date,and we have also detected the uncongditional and conditionalQTL for1000-grain weight and gain weight per panicle in three environments in oneyears. Results as below:1QTL mapping for grain weight per panicle18unconditional QTL are detected on11chromosomes(2B、4B、1B、2B、6A2、1A1、3B、4A1、2D、5D and1A) in3genetic populations. The contribution rate ofunconditional QTL that controls grain weight per panicle of RIL1population is51.1%,in RIL2population, it’s40.09%and of DH population, it’s15.76%.In this study8conditional QTL which control grain weight per panicle aredetected. Only one new QTL is detected between DAA25-MT, Qgwp6A-32, of RIL1population, its contribution rate is4.80%. Other7QTL are detected on DAA15ofthree genetic populations. Their total contribution rate is39.97%, the effect of theseQTL is the cumulative effects of QTL/gene which control grain weight per panicle15days after flowering.2QTL mapping for1000-grain weight22unconditional QTL are detected on9chromosomes(1B、3B、6A、6B、2D、4A、7B、5D and1A).Same QTLs are detected on chromosome1B and3B both ofRIL1population and RIL2population. Same QTL is detected on chromosome6A both of RIL1population and DH population. Same QTL is detected on chromosome2Dboth of RIL2population and DH population.In this study, we have detected10conditional QTL.5conditional QTL aredetected of DH population which control1000-grain weight,2new QTL are detectedbetween DAA15-DAA25, Qtgw2D-4and Qtgw5B, their contribution rate is5.93%and3.11%respectively. Qtgw2D-11which located on chromosome2D is detectedbetween DAA25-MT, its contribution rate is8.23%. Only2QTL which control1000-grain weight are detected of RIL1population, Qtgw1B-15is detected onDAA15, its contribution rate is3.66%, a new QTL is detected between DAA25-MT,its contribution rate is6.76%.3QTL are detected of RIL2population, Qtgw2D-8isdetected on DAA15, Qtgw2D-11和Qtgw3B-1are detected between DAA15-DAA25,its contribution rate is2.24%and8.60%respectively.3QTL mapping for heading dateUnder3different environments,8QTL are detected in3different geneticpopulations which control heading date.3QTL are detected of DH population whichcontrol heading date, among them, contribution rate of Qhd5D-10can be19.66%, it isthe major QTL.4QTL are detectedof RIL1population which control heading date,among them, contribution rate of Qhd1B-32on chromosome1B is biggest, it is themajor QTL. Only1QTL on chromosome1B is detected of RIL2population, itscontribution rate is2.58%. All of Qhd1B-32detected of RIL1population, Qhd1B-26detected in RIL2population and Qhd1B-2detectedof DH population are onchromosome1B.4QTL mapping for flowering date7QTL are detected in3different geneticpopulations which control floweringdate. Among them, of DH population,3QTL are same both in heading date andflowering date. Contribution rate of Qfd1B-3is2.58%and5.94%,contribution rate ofQfd2A-16is2.66%and2.70%,contribution rate of Qfd5D-10is22.79%and19.66%.