MingQiong Platform Wild Camellia Germplasm Genetic Diversity Of DNA Molecular Markers

Camellia oleifera, belong to Theaceae, which is one of the four major woody oil crops in the world. Tea oil is beneficial to human health and be suitable for long-term consumption. Camellia Oleifera germplasm resources in China are mainly distributed in Hunan, Jiangxi, Guangxi, Zhejiang, Fujian, Sichuan etc. PuJiang-Qionglai-Mingshan area in Sichuan (Mingqiong platform) has very rich resources of wild Camellia for a long time. However, these resources do not be managed and preserved properly. The reserach on the genetic diversity of Camellia oleifera resources was great benefit to the breeding and cultivation. In recent years, molecular marker in plant genetic diversity has been widely used in many researches. In this study,65Camellia Oleifera were investigated by ISSR, SRAP and RAPD markers, then which were analysed through a clustering tree. And some certain theoretical consequences were obtained. The results are as follows:1Optimization for ISSR,SRAP and RAPD reaction systemThe best ISSR-PCR system was:Mix12.5μL2PCR, DNA template solution1.25μL, primer1.25μL (10pmol/L), ddH2O up to25μL, annealing temperature for the52-58℃. The best SRAP-PCR system was:Mix12.5μL2PCR, DNA template solution1μL, primer1μL (10pmol/L), ddH2O up to25μL, annealing temperature for the50℃. The best ISSR-PCR system was:Mix12.5μL2PCR, DNA template solution1pL, primer1μL (10pmol/L), ddH2O up to25μL, annealing temperature for the36.4-41℃.216high-polymorpHism ISSR primers were screened out of60ISSR primers,19from143SRAP primers, and8from30RAPD primers. Temperature gradient method was used to identify the optimal annealing temperature.213polymorphic locus were amplified by ISSR primer,281by SRAP primer, and105by RAPD primer.3The clustering results show that Wild Camellia from Mingqiong platform possesses rich genetic diversity, large genetic differences. From this point, Wild Camellia from Mingqiong platform should be recommended as breeding matierals as its strong genetic improvement potential and sufficient resources.The amplifying results of ISSR, SRAP and RAPD on65Camellia materials were analysed by NTSYsPc-2.1, and genetic relationship clustering tree was formed by UPGMA method. All of the65Camellia were divided into5groups by ISSR,6groups by SRAP, and5groups by RAPD.5. The clustering analysis, which mergered ISSR, SRAP and RAPD by statistics, showed that the similarity coefficient of65Camellia germplasms were from0.62to0.94. And all the tested materials were divided into5clusters. The consistency test showed that the clustering results of ISSR molecular markers were more similar to the combined clustering results.