Study On Characteristics Of Microbial Communities In The Process Of Biological Hydrogen Production

This paper is supported by the National Natural Science Foundation (No.2012AA051502).Photosynthetic hydrogen production by microbial growth process and metabolicmechanisms as a biological hydrogen production system research content, a directimpact on the energy consumption of the hydrogen production system, the activity ofhydrogen production, hydrogen production rate and other factors, which restricts thephotosynthetic organisms hydrogen production industrial development. Paperphotosynthetic hydrogen production microflora as the object of study, single factorinfluencing factors and orthogonal experiment method, photosynthetic hydrogenproduction microflora growth under different pH, temperature, inoculum size, lightintensity and culture conditionsconditions, analysis of photosynthetic bacteria growthcharacteristics of various culture conditions and the establishment of logistic growthkinetics modeling flora growth curve, and discuss the matrix with differentconcentrations of glucose for hydrogen production, hydrogen production conditions,the growth of photosynthetic bacteria and hydrogen production, aimed at biologicalhydrogen production from microbial growth studies provide reference and theoreticalbasis. The study concluded that:(1) The PSB group growth conditions in the different culture conditions, and theoptimal growth conditions of the flora. The results showed that: photosynthetichydrogen production microflora growth kinetics model the Logistic equation can beused to simulate, model experimentally measured maximum specific growth rate,photosynthetic bacteria actual curve of the growth process and model of logisticgrowth curve fitting0.9813, model can be used to describe the growth of the PSBgroup; primary and secondary relationship of temperature, inoculum size, lightintensity and pH and other factors on the growth of PSB as follows: pH value, theillumination, inoculation, wherein the pH value is the most significant impact factor.(2) The growth of photosynthetic bacteria and hydrogen production of smallmolecules such as acetic acid, butyric acid, peptone alkyd as carbon source, different carbon sources and the same carbon source at different concentrations of cell growthand hydrogen production has some impact, PSB group with acetic acid as hydrogenproduction substrate, the optimum concentration for3g/L.(3) Photosynthetic hydrogen production by mixed acid and alcohol as a matrix ofgrowth carbon and hydrogen production to take advantage of acetic acid, butyric acid,lactic acid and other small molecules. The PSB group with acetic acid as the substratehydrogen production, the optimum concentration for3g/L, this condition bacterialhydrogen production activity of the highest production of hydrogen.(4) Combined with the law of cell growth in the process of hydrogen productionand hydrogen production rate, analyzing the correlation of the percentage of thehydrogen production rate and mixed cultures of bacteria at different times, toconclude: in the process of hydrogen production, hydrogen production ability of thedominant strain of R. rubrum and R. palustris the capsular rhodopseudomonashydrogen production center, the capsulatus Comedy auxiliary hydrogen-producingbacteria, hydrogen-producing ability to contribute to. poor.